Preparation of pillar[5]arene immobilized trypsin and its application in microwave-assisted digestion of Cytochrome c

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dc.contributor.authorAtacan, Keziban
dc.contributor.authorKursunlu, Ahmed Nuri
dc.contributor.authorOzmen, Mustafa
dc.date.accessioned2020-03-26T20:19:10Z
dc.date.available2020-03-26T20:19:10Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Fen Fakültesi, Kimya Bölümüen_US
dc.description.abstractThis work presents the immobilization of trypsin on the synthesized pillar[5]arene derivative (P5) containing ten iodo-terminals. The characterization has been carried out by Thermogravimetric Analysis (TGA), Fourier Transform Infrared (FTIR) and Raman spectra, Scanning Electron Microscopy (SEM) and Zeta Potential. Furthermore, Cytochrome c (Cyt c) was chosen as a model protein for evaluation of the performance of the pillar [5]arene-immobilized trypsin (P5-T), and its microwave-assisted digestion conditions were investigated by using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for 15 second. The peptide numbers of 10 and sequence coverage of 93% for 15 second microwave-assisted digestion were obtained for P5-T. The results can be further applied in future proteomics studies due to high efficiency, good reproducibility and stability of the pillar[5]arene-immobilized trypsin.en_US
dc.description.sponsorshipScientific Research Projects Commission of Sakarya UniversitySakarya University [2016-02-04-047]en_US
dc.description.sponsorshipThe authors thank Prof. J.-F. Nierengarten and Dr. I. Nierengarten for laboratory studies and scientific comments. The authors would like to thank Prof. Dr. Mahmut Ozacar for his support. The authors also would like to appreciate the kind help to Assoc. Prof. Dr. Bunyemin Cosut for MALDI-MS analyses. This work is financed by the Scientific Research Projects Commission of Sakarya University (Project number: 2016-02-04-047) and is gratefully acknowledged.en_US
dc.identifier.citationAtacan, K., Kursunlu, A. N., Ozmen, M. (2019). Preparation Of Pillar [5] Arene İmmobilized Trypsin And İts Application İn Microwave-Assisted Digestion Of Cytochrome C. Materials Science And Engineering: C, 94, 886-893.
dc.identifier.doi10.1016/j.msec.2018.10.043en_US
dc.identifier.endpage893en_US
dc.identifier.issn0928-4931en_US
dc.identifier.issn1873-0191en_US
dc.identifier.pmid30423776en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage886en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.msec.2018.10.043
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38120
dc.identifier.volume94en_US
dc.identifier.wosWOS:000451360300088en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKursunlu, Ahmed Nuri
dc.institutionauthorOzmen, Mustafa
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofMATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectPillar[5]areneen_US
dc.subjectTrypsinen_US
dc.subjectImmobilizationen_US
dc.subjectCytochrome cen_US
dc.subjectMicrowave digestionen_US
dc.titlePreparation of pillar[5]arene immobilized trypsin and its application in microwave-assisted digestion of Cytochrome cen_US
dc.typeArticleen_US

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