Plasmid-mediated fluoroquinolone resistance in clinical isolates of escherichia coli in Konya, Turkey
Yükleniyor...
Dosyalar
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
CUKUROVA UNIV, FAC MEDICINE
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Purpose: Resistance to quinolones usually results from mutations in the topoisomerase genes encoded chromosomally and also the expression of efflux pumps, loss of porines and the transfer of plasmid-mediated genes. The aim of this study was to investigate the presence of plasmid-mediated quinolones resistance genes (qnrA, qnrB, qnrC, qnrS, qepA, and aac(6')-1b-cr) in clinical isolates of Esherichia coli from Selcuk University, Konya, Turkey. Materials and Methods: In this study 115 quinolone-resistant isolates were screened for qnrA, qnrB, qnrC, qnrS, qepA, and aac(6')-1b-cr genes by polymerase chain reaction (PCR). All aac(6')-1b positive amplicons were analyzed by digestion with BseGI restriction enzyme to identify aac(6')-1b-cr variant. Results: Of the 115 quinolone-resistant E. coli strains, three (2.6%) carried qnrB, nine (7.8%) carried qnrS and 50 (43.5%) carried aac(6')-1b-cr genes. None of them harboured qnrA, qnrC and qepA genes. Conclusion: We determined that aac(6')-1b-cr gene was responsible for most of the quinolone-resistant E. coli strains from Konya, Turkey. The prevalence of qnrB and qnrS genes was low and qnrA, qnrC and qepA genes were not detected. The surveillance of quinolone resistance genes is important, especially plasmid mediated ones are rapidly spreading all over the world.
Açıklama
Anahtar Kelimeler
Escherichia coli, plasmid-mediated fluoroquinolone resistance, qnr, qepA, aac(6 ')-1b-cr
Kaynak
Cukurova Medical Journal
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
43
Sayı
2
Künye
Azeez, D. A., Findık, D., Dağı, H. T., Arslan, U. (2018). Plasmid-mediated fluoroquinolone resistance in clinical isolates of Escherichia coli in Konya, Turkey. Cukurova Medical Journal, 43(2), 295-300.
