Growth-Promoting Effects of Different Fractions of Extra-Embryonic Coelomic Fluid on Embryonic Development

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dc.contributor.authorKarabulut, A. K.
dc.contributor.authorLayfield, R.
dc.contributor.authorPratten, M. K.
dc.date.accessioned2020-03-26T16:36:15Z
dc.date.available2020-03-26T16:36:15Z
dc.date.issued2000
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn the early stages of embryonic development, many growth-promoting molecules must be provided by the maternal system. These factors may be supplied locally to the embryo, by the decidua, the placenta, or the yolk sac. In this study the growth-promoting potential of extra-embryonic coelomic fluid (EECF) and its fractions was investigated. The embryonic requirement of growth-promoting molecules may be studied by reducing the growth-supporting capacity of serum. Thus, ultrafiltration of rat serum was carried out for 8h using Millipore filters with a molecular weight exclusion of 30 kDa. Rat embryos at 9.5 days of age were cultured for 8 days for anembryonic yolk sacs, and then EECF was collected and divided into three different molecular weight fractions by ultrafiltration. Rat embryos were cultured for 48 h in whole rat serum and the serum retenate (which has low growth-supporting capacity) in the presence and absence of EECF, its fractions, or in EECF only. Embryos grown in retenate showed severe growth retardation, and the addition of EECF significantly improved embryonic growth. The fraction which contained the molecules with molecular weight between 10 and 30 kDa had significantly more effect on embryonic development than the other fractions. This fraction of EECF was analysed by gel electrophoresis. Three of the four protein bands observed in this fraction were identified by amino-terminal sequencing as alpha-fetoprotein precursor (22 kDa), apolipoprotein Al precursor (24kDa) and fetal haemoglobin Y2 chain /14kDa), none of which are likely to be responsible for the growth-promoting activity. To further investigate growth-promoting proteins, EECF was Western-blotted to nitrocellulose membranes and probed with antisera against rat prolactin, epidermal growth factor, insulin-like growth factors I and II and human placental lactogen. No immunoreactive bands were detected in the EECF, suggesting that either these proteins are not present or are present at levels too low to be detected. Although the growth-promoting effect of the EECF was demonstrated in this study, the molecules responsible remain uncharacterized.en_US
dc.identifier.citationKarabulut, A. K., Layfield, R., Pratten, M. K., (2000). Growth-Promoting Effects of Different Fractions of Extra-Embryonic Coelomic Fluid on Embryonic Development. Anatomia Histologia Embryologia, 29(4), 225-234. Doi: 10.1046/j.1439-0264.2000.00269.x
dc.identifier.doi10.1046/j.1439-0264.2000.00269.xen_US
dc.identifier.endpage234en_US
dc.identifier.issn0340-2096en_US
dc.identifier.issn1439-0264en_US
dc.identifier.issue4en_US
dc.identifier.pmid11008370en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage225en_US
dc.identifier.urihttps://dx.doi.org/10.1046/j.1439-0264.2000.00269.x
dc.identifier.urihttps://hdl.handle.net/20.500.12395/17217
dc.identifier.volume29en_US
dc.identifier.wosWOS:000089133700007en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKarabulut, A. K.
dc.language.isoenen_US
dc.publisherWıleyen_US
dc.relation.ispartofAnatomia Histologia Embryologiaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleGrowth-Promoting Effects of Different Fractions of Extra-Embryonic Coelomic Fluid on Embryonic Developmenten_US
dc.typeArticleen_US

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