Pretreatment of Candida rugosa lipase with soybean oil before immobilization on beta-cyclodextrin-based polymer
Küçük Resim Yok
Tarih
2009
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
ELSEVIER SCIENCE BV
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, Candida rugosa lipase was pretreated with soybean oil in order to allow fatty acids to bond to the active site before immobilization. The pretreated C rugosa lipase was immobilized on beta-cyclodextrin-based polymer by crosslinking with hexamethylene diisocyanate (HMDI). This pretreated lipase exhibited steric hindrance around the active site such that during immobilization, covalent bonds were formed between the carrier and the lipase region far from the active site. The pretreated lipase was immobilized on a beta-cyclodextrin-based polymer by crosslinking with hexamethylene diisocyanate with the activity of 42 U/g-matrix, which is 5.7 times higher than that of the immobilized non-pretreated lipase. In addition, the immobilized lipase activity was maintained at levels exceeding 60% of its original activity after 10 reuses. (c) 2008 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Lipase immobilization, Candida rugosa lipase, beta-Cyclodextrin, Soybean oil
Kaynak
COLLOIDS AND SURFACES B-BIOINTERFACES
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
69
Sayı
1
