Pretreatment of Candida rugosa lipase with soybean oil before immobilization on beta-cyclodextrin-based polymer

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dc.contributor.authorOzmen, Elif Yilmaz
dc.contributor.authorYilmaz, Mustafa
dc.date.accessioned2020-03-26T17:39:46Z
dc.date.available2020-03-26T17:39:46Z
dc.date.issued2009
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn this study, Candida rugosa lipase was pretreated with soybean oil in order to allow fatty acids to bond to the active site before immobilization. The pretreated C rugosa lipase was immobilized on beta-cyclodextrin-based polymer by crosslinking with hexamethylene diisocyanate (HMDI). This pretreated lipase exhibited steric hindrance around the active site such that during immobilization, covalent bonds were formed between the carrier and the lipase region far from the active site. The pretreated lipase was immobilized on a beta-cyclodextrin-based polymer by crosslinking with hexamethylene diisocyanate with the activity of 42 U/g-matrix, which is 5.7 times higher than that of the immobilized non-pretreated lipase. In addition, the immobilized lipase activity was maintained at levels exceeding 60% of its original activity after 10 reuses. (c) 2008 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.colsurfb.2008.10.021en_US
dc.identifier.endpage62en_US
dc.identifier.issn0927-7765en_US
dc.identifier.issn1873-4367en_US
dc.identifier.issue1en_US
dc.identifier.pmid19091527en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage58en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.colsurfb.2008.10.021
dc.identifier.urihttps://hdl.handle.net/20.500.12395/23784
dc.identifier.volume69en_US
dc.identifier.wosWOS:000263852900008en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofCOLLOIDS AND SURFACES B-BIOINTERFACESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectLipase immobilizationen_US
dc.subjectCandida rugosa lipaseen_US
dc.subjectbeta-Cyclodextrinen_US
dc.subjectSoybean oilen_US
dc.titlePretreatment of Candida rugosa lipase with soybean oil before immobilization on beta-cyclodextrin-based polymeren_US
dc.typeArticleen_US

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