An assessment of the nutraceutical potential of Juglans regia L.leaf powder in diabetic rats
Küçük Resim Yok
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
PERGAMON-ELSEVIER SCIENCE LTD
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, we evaluated the nutraceutical potential of juglans regia L (a dietary supplement and food additive) by evaluating the in-vitro anti-diabetic potential and by assessing the in-vivo anti hyperglycaemic, anti-hyperlipidaemic, and organ-protective effects of freshly-dried and powdered leaves of J. regia L in diabetic rats. In the in-vivo experiments, dry powder of J. regia L leaf (25, 50 and 100 mg/kg) was administered orally, twice daily (9.00 a.m. and 5 p.m.) to streptozocin-induced diabetic rats over a period of 28 days, during which body weight and blood glucose were monitored weekly. At the end of the experimental period, animals were sacrificed, blood was taken for assessment of lipid profile, antioxidant activity and liver/kidney biochemistry; while samples of the pancreas, liver and kidneys were fixed, processed, sectioned, and stained for general histology. Phytochemical evaluations of three extracts were carried out using HPLC-PDA validated procedures, while enzyme-inhibitory potentials were tested against alpha-amylase and alpha-glucosidase. In-vivo assays showed that twice-daily administration of J. regia L leaf resulted in weight gain, glycaemic control, reversal of dyslipidaemia and biochemical evidences of liver/kidney injury, and protection against pancreas, liver and kidney tissue injury. (C) 2017 Elsevier Ltd. All rights reserved.
Açıklama
Anahtar Kelimeler
HPLC-PDA, Polyphenols, Flavonoids, Juglans regia L, Diabetes
Kaynak
FOOD AND CHEMICAL TOXICOLOGY
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
107
