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Öğe Ankara keçilerinin genetik yapılarının nişasta jel elektroforezi yöntemiyle araştırılması(2007) Altunok, Vahdettin; Nizamlıoğlu, Mehmet; Bulut, ZaferBu çalışma Ankara keçilerinin genetik yapılarını belirlemek ve bu keçiler ile diğer keçi ırkları arasındaki benzerlikleri (yada farklılıkları) ortaya koymak için yapıldı . Bu amaçla süperoksit dismutaz (SOO), karbonik anhidraz 1 (CA1), esleraz D (EsD), glutamat okzaloasetat transaminaz (GOT), fosfoglukonat dehidrogenaz (PGO) enzimlerinin lokusun işasla jel elektroforezi ile araştırılmıştır. Araştırmada "Yerköy Hayvancılık Araştırma Enstitüsü (Yozgat)'nden 100 baş Ankara keçisi ve diğer 3 keçi ırkı (Saanen, kıl ve Malla keçisi)'ndan 19 keçinin alyuvar enzimleri kullanılmıştır. Her enzim için literatür verilerine uygun tampon ve enzim aktivite boyaması için ise özel kimyasallar kullanılmıştır. Gözlenen izoenzim bantları analiz edilmiştir. Sod, Ca1, EsO, Got ve Pgd enzim lokusları yönünden, Ankara keçilerinde ve diğer keçilerde varyasyon görülmemiştir. Ankara ve diğer keçilerin aynı bantlara sahip ve hepsinin homozigot olduğu görülmüştür. Bu çalışma ile Ankara keçilerinde çalışılan 5 enzim lokusunun monomorfik ve keçilerin hepsinin homozigot olduğu, ayrıca Ankara keçilerinin araştırılan lokuslar açısından çalışılan d iğer keçi ırkıarından farklı bir allel taşımadığı ortaya konmuştur.Öğe Biyolojik saatin moleküler temeli(2005) Eroğlu, Tekin; Altunok, VahdettinÖzet: Sirkadian ritimler veya biyolojik saat 24 saatlik periyotlarda etkili olaylardır. Sirkadian ritimler birçok biyokimyasal düzeylerde canlı organizmasının, davranışlarını, fizyolojilerini ve fonksiyonlarını düzenler. Bu ritimler kan basıncını, vücut ısısını ve hormon düzeylerini içerir. Bu saatin anormal çalışması depresyon ve uykusuzluk başta olmak üzere zeka ve fiziksel faaliyetlerde aksaklıklara neden olabilir. Bu derlemede biyolojik ritimler ve biyolojik saatin moieküler mekanizması ele alınmıştır.Öğe Blood and Colostrum/Milk Serum Gamma-Glutamyltransferase Activity as a Predictor of Passive Transfer Status in Lambs(Blackwell Verlag Gmbh, 2003) Maden, Mehmet; Altunok, Vahdettin; Birdane, Fatih Mehmet; Aslan, V.; Nizamlıoğlu, MustafaThe importance of blood and colostrum/milk serum gamma-glutamyl transferase (gamma-GT) enzyme activity was evaluated to assess passive transfer status in healthy lambs. Thirty Akkaraman sheep (3-6 years old) were used which had normal pregnancy period and the same conditions, and the age of the lambs ranged between 0 and 15 days. Blood and colostrum/milk samples were collected from sheep and lambs after birth, before suckling (0) and after on 1st, 3rd, 7th and 15th days. Serum immunoglobulin G (IgG) concentration was determined by the use of Single Radial Immunodiffusion method. Serum gamma-GT activity was measured, using a commercially available kit in blood and colostrum/milk samples. Correlations were carried out between immunoglobulin and gamma-GT levels. Regression models (simple and multiple) were calculated with significant data. Linear correlation was determined between colostrum/milk gamma-GT activity and IgG concentrations and between serum gamma-GT activity and IgG concentrations in lambs on the 0 day. (r: 0.607, P: 0.001), 1st (r: 0.768, P: 0.001) and the 3rd (r: 0.603, P: 0.001) days and on the 1st (r: 0.637, P: 0.001) and 3rd (r: 0.478, P: 0.012) days in the experiment, respectively. Multivariate regression models were developed to estimate sample IgG concentration. Serum and colostrum/milk IgG concentration could be predicted using the formula: lamb serum IgG = 825 + 0.688 (lamb gamma-GT) + 52 (days); colostrum/milk IgG = 832 + 0.505 (colostrum/milk gamma-GT) - 167 (days). The regression models were moderately accurate in predicting serum IgG concentration (R-2 = 0.51) and colostrum/milk IgG concentration (R-2 = 0.55). Test sensitivity and positive predictive values for serum gamma -GT enzyme activity were found to be 96 and 100% and for colostrum/milk gamma-GT enzyme activity were found to be 100 and 68% to prediction IgG concentration. Serum and colostrum/milk gamma-GT activity can be used to assess passive transfer status of lambs. Along with this, regression models used to calculate serum and colostrum/milk gamma-GT activities found to be useful to estimate sample IgG concentration. The use of serum and colostrum/milk gamma-GT enzyme activity was found useful especially after birth on the 0, 1st and 3rd days.Öğe Broiler Civcivlerde Yeme Yalnız ve Kombine Katılan Aflatoksin ve Adsorban (Polivinilpolipirolidon)'in Bazı Kan Değerleri Üzerine Etkileri(Selçuk Üniversitesi, 1996) Oğuz, Halis; Altunok, Vahdettin; Demet, Ömer; Nizamlıoğlu, FarhanThis study was carned out to investigate the effect of aflatoxin(produced by Aspergillus parasitıcus NRRL 2999 on rice) that was added alone and its combination with adsorbent (polyvlnylpolypyrrolidone) on some ha· ematologıcaı values a day-old in eighty broiler chicks (Avian). For this purpose, the animals, which were divided into four groups, were provided with feed and water for ad libitum consumptlon from 1 to 28 days of age. The experirnental design consisted of four dietary treatments in the feed of the groups: a) Control with O mg of aflatoxın (AF) per kg of diet. b) 2. 5 mg of AF per kg of diet, c) 3. O g of polyvinylpolypyrrolidone (PVPP) per kg of dlet d) 2. 5 mg of AF per kg of diet plus 3 o g of PVPP per kg of dieı. AF that were incorporated to diet consisted of 83. 06 % B ı, 12. 98 % B2, 2. 84 % G 1, and 1. 12 % G2. At the e nd of the experiment. s ix chlcks per each group w ere bl ed by cardıac puncture and ıheır blood samples were collected and analysed for treated-related changes In serum glucose, total protein, albumin, bilirubin and aspartate aminotransferase (AST) enzım activity. These results lndlcate that aflatoxin caused a deereasa on serum total protein, albumin, and bilırubın values (p>O. 05), and its eHects on glucose values and AST activity were not appear Also the inert polymer (polyvinıylpolypyrrolidone) incorporated to diet 3. O g/kg of feed, has no benetilical effect on aflatoxin contaminated (2. 5 ppm) feed for these paramaters on broilers.Öğe Ci?vci?vlerde Poli?klorobifenil (PCB) Bi?leşi?ği?ni?n Karaci?ğer Yağlanması ve Bazı Kan Değerleri? Üzeri?ne Etki?si?(Selçuk Üniversitesi, 1995 Ocak) Nizamlıoğlu, Mehmet; Eren, Ülker; Altunok, Vahdettin; Boydak, MuratIn this present study, the effect of polychlorinated biphenyl (PCB) on fatty liver and some blood parameters in chickens were studied. For this purpose,32 egg type male chickens at 1 day of age obtained from a commercial hatchery were used as materials. Animals were divided into 4 equal groups. First group was keept as control group. The other groups were used as treatment group 1,2,3, and administrated 5,10,30 mg/kg of PCB with food respectively. Animals were fed and watered ad libitum during the experiment 40 days. End of the experiment, blood samples were collected heart puncture and after cutting all of the animals liver samples were taken immediately. Blood serum cholesterol, triglycerides and AST values were determined spectrophotometrically (Shimadzu 2100), Na and K levels were performed with Flame Photometer Plasma vitamin A and vitamin E values were measured with HPLC. The lipid infiltrations in the cytoplasma of liver paranchymal cells in control, treatment 2 and 3.groups were mild, but the other treatment group was moderate. Cholesterol, triglycerides, AST, vitamin A and vitamin E values were different from control group and found statistically significant (P<0.05).Na and K levels in the groups were not changed statistically. As a result, the changing of some blood parameters were correlated with the dose level of 5,15,30 mg/kg PCB.The lower levels of PCB were not produced significant fatty liver in chickens.Öğe Concentrations of Some Elements in Blood Serum of Angora Goats(Polish Soc Veterinary Sciences Editorial Office, 2006) Yazar, Enver; Altunok, Vahdettin; Eroğlu, TekinThe aim of the study was to determine serum essential trace, macro and industrial element levels of Angora goats according to breed and age differences. Clinically healthy (ages 2-3 and 4-5) female Angora goats of two different breeds (Eskisehir and Lalaban) were used as material at the Lalahan Livestock Research Institute. Serum aluminum (Al), arsenic (As), boron (B), barium (Ba), cobalt (Co), chromium (Cr), copper (Cu), gallium (Ga), indiurn (In), lead (Pb), lithium (Li), nickel (Ni), selenium (Se), silver (Ag), sulfur (S), strontium (Sr), vanadium (V) and zinc (Zn) were measured with an ICP-OES plasma optical atomic emission spectrometer. There were no statistically significant differences found in the different breeds and age groups.Öğe Concentrations of total serum immunoglobulin e, a, g and m in stray dogs with healthy and dermatological problems(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2013) Maden, Mehmet; Birdane, Fatih Mehmet; Ucan, Uckun Sait; Altunok, VahdettinIn this study, clinical and laboratory findings along with serum IgE, IgA, IgM and IgG concentrations were determined in stray dogs (healthy and with dermatological problems). It was determined that skin lesions were localized in the rate of 69.8% (n=44) and generalized in the rate of 30.2% (n=19) of sick animals. Serum IgE concentration in stray dogs with dermatologic problems was significantly higher (P<0.001) than that of healthy dogs whereas serum IgM and G concentrations were significantly lower (P<0.05). It was evaluated that, serum total immunoglobulin concentrations could be useful in making contribution to differential diagnosis of skin diseases in stray dogs.Öğe Effect of flunixin meglumine on cytokine levels in experimental Endotoxemia in mice(WILEY, 2007) Yazar, Enver; Er, Ayşe; Üney, Kamil; Altunok, Vahdettin; Elmas, MuammerIn this study, effect of flunixin meglumine on serum tumour necrosis factor alpha, (TNF alpha) interleukin-1 beta and interleukin-10 levels was investigated in lipopolysaccharide-induced endotoxic mice. Healthy 273 Balb/C mice were used and divided into three equal groups. Group 1 was injected lipopolysaccharide (Escherichia coli 0 111:134, 250 mu g/mouse, intraperitoneally), Group 2 was injected flunixin meglumine (2.5 mg/kg, subcutaneously), and Group 3 was injected lipopolysaccharide + flunixin meglumine. After the treatments, at 0., 1., 2., 3., 6., 12., 24th hours and 3., 5., 7. 14., 21., 28th days blood samples were taken from seven mice in each group. Serum TNF alpha, interleukin-1 beta and interleukin-10 levels were measured using commercially available kits by enzyme-linked immunoassay. Flunixin meglumine did not affect the cytokine levels in healthy animals. While lipopolysaccharide increased serum TNF alpha, interleukin-1 beta and interleukin-10 levels, flunixin meglumine inhibited increases at levels of all cytokines. As result, flunixin meglumine showed depressor effect on cytokine levels in endotoxemia and the effect may be a reason for the first chosen member of nonsteroid anti-inflammatory drug in endotoxemia.Öğe Effect of Phenobarbital on Antioxidant Enzyme Activities and Blood Gas Parameters in Balb/C Mice(ECOLE NATIONALE VETERINAIRE TOULOUSE, 2001) Demir, O.; Yazar, Enver; Altunok, Vahdettin; Elmas, Muammer; Özdemir, VuralIn this study, effect of Phenobarbital was investigated on antioxidant enzyme activities and blood gas parameters in Balb/C mice. Forty male Balb/C mice were used. Ten mice were served as a control group, and thirty mice were administered phenobarbital (80 mg/kg body weight, orally, single administration). Blood, brain and liver samples were taken at 6, 12 and 24 hours after administration. Brain and liver tissues superoxide dismutase and glutathione peroxidase activities were measured by spectrophotometry, and blood gas parameters were measured with blood gas analyzer. As results, phenobarbital caused temporary respiratory acidosis and a decrease in brain tissue superoxide dismutase activity.Öğe Effect of Tilmicosin on Cardiac Muscle and Serum Creatine Kinases Activities and Serum Total Protein Level in Healthy Male Balb/C Mice(ECOLE NATIONALE VETERINAIRE TOULOUSE, 2001) Yazar, Enver; Altunok, Vahdettin; Elmas, Muammer; Traş, Bünyamin; Baş, Ahmet Levent; Özdemir, VuralIn this study, the effect of tilmicosin on cardiac muscle and serum creatine kinases activities and serum total protein level was investigated. Forty male Balb/C mice were used as materials. Ten mice were used as a control group, and thirty mice were injected with tilmicosin (25 mg/kg body weight, SC, single injection) and monitored for 3 days. The results obtained in the present study show that use of tilmicosin caused temporary increases in cardiac muscle creatine kinase activity and serum total protein level in male Balb/C mice.Öğe The Effect of Tilmicosin on Cardiac Superoxide Dismutase and Glutathione Peroxidase Activities(Blackwell Verlag Gmbh, 2002) Yazar, Enver; Altunok, Vahdettin; Elmas, Muammer; Traş, Bünyamin; Baş, Ahmet Levent; Özdemir, V.In this study, the effect of tilmicosin on cardiac superoxide dismutase and glutathione peroxidase activities was investigated. Forty male BALB/c mice were used as material. Ten mice served as a control group, and 30 mice were injected with tilmicosin (25 mg/kg body weight. subcutaneously. with a single injection). After drug administration, they were monitored for 3 days. Tilmicosin caused decreases in cardiac superoxide dismutase and glutathione peroxidase activities.Öğe Effects of Aminoglycoside Antibiotics on Renal Antioxidants, Malondialdehyde Levels, and Some Serum Biochemical Parameters(Canadian Vet Med Assoc, 2003) Yazar, Enver; Elmas, Muammer; Altunok, Vahdettin; Sivrikaya, Abdullah; Öztekin, Esma; Birdane, Yavuz OsmanEffects of amikacin, gentamicin, kanamycin, and streptomycin on renal tissue superoxide dismutase, glutathione peroxidase, glutathione and malondialdehyde, serum creatinine, potassium, sodium, total protein, glucose, uric acid, and total bilirubin levels were investigated. All aminoglycoside antibiotics decreased renal tissue glutathione levels.Öğe Effects of Continuous Supplementations of Ascorbic Acid, Aspirin, Vitamin E and Selenium on Some Haematological Parameters and Serum Superoxide Dismutase Level in Broiler Chickens(Carfax Publishing, 2000) Traş, Bünyamin; İnal, Fatma; Baş, Ahmet Levent; Altunok, Vahdettin; Elmas, Muammer; Yazar, Enver1. This study was conducted using male broiler chickens to determine the effects of ascorbic acid, aspirin, ascorbic acid+aspirin, vitamin E+selenium and ascorbic acid+aspirin+vitamin E+selenium supplementations on haematological parameters and serum superoxide dismutase concentration. 2. One hundred and twenty day-old male Hubbunt broiler chicks were randomly divided into 6 experimental groups of 20 chicks each and placed in different pens. Groups 2, 3, 4, 5 and 6 were given a diet supplemented with ascorbic acid, aspirin (in water), ascorbic acid+aspirin, vitamin E+selenium and ascorbic acid+aspirin+vitamin E+selenium, respectively for 45 d while group 1 was given a commercial broiler diet. 3. There was no significant effect of ascorbic acid, aspirin, ascorbic acid+aspirin, vitamin E+selenium supplementations on any of the haematological parameters (red blood cell, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin concentration, mean corpuscular haemoglobin) in broilers but ascorbic acid+aspirin+vitamin E+selenium supplementation significantly decreased the white blood cell counts. 4. In addition to this, ascorbic acid, aspirin, ascorbic acid+aspirin and ascorbic acid+aspirin+vitamin E+selenium supplementations had no significant effect on the serum superoxide dismutase level, but vitamin E+selenium supplementation increased the serum superoxide dismutase level.Öğe Effects of Continuous Supplementations of Ascorbic Acid, Aspirin, Vitamin E, and Selenium on Performance, Immune Response, and Some Biochemical Parameters Under Normal Environmental and Management Conditions in Broilers(EUGEN ULMER GMBH CO, 2001) Traş, Bünyamin; İnal, Fatma; Baş, Ahmet Levent; Altunok, Vahdettin; Elmas, Muammer; Yazar, EnverMale broiler chickens were used to determine the effects of ascorbic acid, aspirin, ascorbic acid + aspirin, vitamin E + selenium, and ascorbic acid + aspirin + vitamin E + selenium supplementation on performance, IgG levels, and some biochemical parameters in blood. Three hundred and ninety six 1-day-old male. Hubbunt broilers were randomly divided into six groups. Each group was placed in different pens and feed and water were provided ad libitum. Performance parameters (body weight, feed intake and feed conversion), IgG levels, vitamins A and E, and some biochemical parameters in blood (Na, K, Cl, total protein and albumin) were measured on days 15, 30, and 45 in all groups. The results revealed at the end of the study showed that aspirin, vitamin C, vitamin E + selenium administrations exhibited no effects on levels of vitamin A, vitamin E, total protein, albumin, Na, Cl and K in blood, while aspirin administration decreased IgG level. Vitamin C administration increased IgG level in serum, aspirin + vitamin C administration increased albumin and K in serum, aspirin + vitamin C + vitamin E + selenium administration increased vitamin E and Na level in serum. Also, there was no significant difference in performance between the control and the experimental groups. It was suggested that vitamin C administration to diets may be useful for broiler breeds due to the observed increased IgG level.Öğe Efficacy of oral rehydration therapy solutions containing sodium bicarbonate or sodium acetate for treatment of calves with naturally acquired diarrhea, moderate dehydration, and strong ion acidosis(AMER VETERINARY MEDICAL ASSOC, 2009) Sen, Ismail; Altunok, Vahdettin; Ok, Mahmut; Coskun, Alparslan; Constable, Peter D.Objective-To determine and compare the effects of 4 oral replacement therapy (ORT) solutions on acid-base balance, abomasal emptying rate, and plasma volume expansion in calves with naturally acquired diarrhea and moderate dehydration. Design-Prospective study. Animals-20 calves. Procedures-20 calves up to 45 days of age were randomly allocated (n = 5/group) to receive 2 L of 1 of 4 treatments via oroesophageal intubation: sodium bicarbonate (150 mmol/L or 300 mmol/L) or sodium acetate (150 mmol/L or 300 mmol/L). The 4 test solutions contained acetaminophen (50 mg/kg [22.7 mg/lb]) and 50 g of glucose monohydrate. Jugular venous blood samples were obtained periodically before and after administration of the ORT solution. Abomasal emptying rate was determined by use of the time to maximal plasma acetaminophen concentration. Results-Plasma bicarbonate concentration increased more rapidly in calves administered bicarbonate-containing ORT solutions, whereas the rate of systemic alkalinization, as assessed via blood pH, did not differ consistently among treatments. The 300 mmol/L ORT solutions were emptied at a significantly slower rate from the abomasum than 150 mmol/L ORT solutions, with no difference in emptying rate between acetate and bicarbonate-containing ORT solutions of similar molality. The 300 mmol/L sodium acetate ORT solution significantly increased plasma volume. Conclusions and Clinical Relevance-Clinically important differences in the resuscitative response to 300 mmol/L or 150 mmol/L ORT solutions of sodium acetate or sodium bicarbonate were not identified. (J Am Vet Med Assoc 2009;234:926-934)Öğe Erythrocyte and spermatozoa glucose-6-nhosphate dehydrogenase activity in merino rams: An experimental study(SHAHID SADOUGHI UNIV MEDICAL SCIENCES, 2018) Gürel, Hüseyin; Başpınar, Nuri; Akalın, Pınar Peker; Altunok, Vahdettin; Kazak, FilizBackground: Glucose-6-phosphate dehydrogenase (G6PD) is the first enzyme of the pentose phosphate metabolic pathway that supplies reducing agents by maintaining the level of reduced nicotinamide adenine dinucleotide phosphate. Objective: It was aimed to determine the activity of erythrocyte and spermatozoa G6PD in the breeding and non-breeding seasons in Merino rams. And also, to find out the relation of these parameters with sperm quality parameters for better understanding the role of this enzyme in male fertility. Materials and Methods: 1.5-2 yr-old healthy, 14 Merino rams were involved. Ejaculate samples were collected using an artificial vagina, in October (the breeding season) and April (the non-breeding season). Blood samples were collected prior to sperm collection. Sperm volume (ml), motility (%), mass activity (1-5), concentration (x10(6)), viability (%), abnormal acrosome morphology (%) and abnormal sperm morphology (%) was evaluated. The activities of spermatozoa and erythrocyte G6PD were determined and the relation of sperm parameters with G6PD activity was evaluated. Results: Erythrocyte G6PD activity was higher (p <= 0.001), whereas spermatozoa G6PD activity was lower (p<0.001) in the breeding season (1.928 +/- 0.231 U/g hemoglobin, 129.65 +/- 28.41 U/g protein, respectively) from that in the non-breeding (0.530 +/- 0.066 U/g hemoglobin, 562.36 +/- 94.92 U/g protein, respectively). There were also significant differences among sperm quality parameters within the seasons. Positive correlation was determined between spermatozoa G6PD activity (r=0.053, p=0.03 and sperm concentration in the breeding season. Conclusion: Higher spermatozoa G6PD activity in October, where the level of polyunsaturated fatty acids is suggested to be increased, may reflect the increased need of nicotinamide adenine dinucleotide phosphate and thus higher G6PD activity for the oxidative balance.Öğe Erythrocyte and spermatozoa glucose-6-phosphate dehydrogenase activity in merino rams: An experimental study(Research and Clinical Center for Infertitlity, 2018) Gürel, Hüseyin; Başpınar, Nuri; Akalın, Pınar Peker; Altunok, Vahdettin; Kazak, FilizBackground: Glucose-6-phosphate dehydrogenase (G6PD) is the first enzyme of the pentose phosphate metabolic pathway that supplies reducing agents by maintaining the level of reduced nicotinamide adenine dinucleotide phosphate. Objective: It was aimed to determine the activity of erythrocyte and spermatozoa G6PD in the breeding and non-breeding seasons in Merino rams. And also, to find out the relation of these parameters with sperm quality parameters for better understanding the role of this enzyme in male fertility. Materials and Methods: 1.5-2 yr-old healthy, 14 Merino rams were involved. Ejaculate samples were collected using an artificial vagina, in October (the breeding season) and April (the non-breeding season). Blood samples were collected prior to sperm collection. Sperm volume (ml), motility (%), mass activity (1-5), concentration (×106), viability (%), abnormal acrosome morphology (%) and abnormal sperm morphology (%) was evaluated. The activities of spermatozoa and erythrocyte G6PD were determined and the relation of sperm parameters with G6PD activity was evaluated. Results: Erythrocyte G6PD activity was higher (p?0.001), whereas spermatozoa G6PD activity was lower (p?0.001) in the breeding season (1.928±0.231 U/g hemoglobin, 129.65±28.41 U/g protein, respectively) from that in the non-breeding (0.530±0.066 U/g hemoglobin, 562.36±94.92 U/g protein, respectively). There were also significant differences among sperm quality parameters within the seasons. Positive correlation was determined between spermatozoa G6PD activity (r=0.053, p=0.03 and sperm concentration in the breeding season. Conclusion: Higher spermatozoa G6PD activity in October, where the level of polyunsaturated fatty acids is suggested to be increased, may reflect the increased need of nicotinamide adenine dinucleotide phosphate and thus higher G6PD activity for the oxidative balance. © 2018, Research and Clinical Center for Infertitlity. All rights reserved.Öğe Evolutionary Relationship Among Three Native and Two Crossbreed Sheep Breeds of Turkey : Preliminary Results(2005) Soysal, M. I.; Koban, Evren; Ünal, Özkan Emel; Altunok, Vahdettin; Bulut, Z.; Nizamlioglu, M.; Togan, I.The Turkish native sheep breeds, possibly being the neighbours of the earliest domesticated sheep populations, might be harbouring important genetic characteristics to be employed in the future for the improvement of sheep breeds. In order to design a conservation strategy, their genetic diversities must be determined. In the present study, based on three microsatellite loci, the genetic diversity of the Kivircik, Awassi, Akkaraman breeds (native) of Turkey as well as two of their crossbreeds Türkgeldi and Konya Merino were studied comparatively. It was observed that their heterozygoties are all high (0.6673-0.7822) compared to previously studied breeds, as expected for populations close to the center of domestication. Neighbour Joining (NJ) tree based on allele sharing distances indicated that the inertia of the breeds are not high. Yet, the genetic differentiations between the breeds based on pairwise FST (inbreeding coefficient) values are all significant. Furthermore, the three microsatellite loci could distinguish three groups of native breeds and their crossbreeds; 1) Awassi, 2) Kivircik-Türkgeldi and 3) Akkaraman-Konya Merino.Öğe Evolutionary relationship among three native and two crossbreed sheep breeds of Turkey: preliminary results(ECOLE NATIONALE VETERINAIRE TOULOUSE, 2005) Soysal, M. İhsan; Koban, Evren; Özkan, Emel; Altunok, Vahdettin; Bulut, Zafer; Nizamlıoğlu, Mehmet; Togan, İnciThe Turkish native sheep breeds, possibly being the neighbours of the earliest domesticated sheep populations, might be harbouring important genetic characteristics to be employed in the future for the improvement of sheep breeds. In order to design a conservation strategy, their genetic diversities must be determined. In the present study, based on three microsatellite loci, the genetic diversity of the Kivircik, Awassi, Akkaraman breeds (native) of Turkey as well as two of their crossbreeds Turkgeldi and Konya Merino were studied comparatively. It was observed that their heterozygoties are all high (0.6673-0.7822) compared to previously studied breeds, as expected for populations close to the center of domestication. Neighbour Joining (NJ) tree based on allele sharing distances indicated that the inertia of the breeds are not high. Yet, the genetic differentiations between the breeds based on pairwise F-ST (inbreeding coefficient) values are all significant. Furthermore, the three microsatellite loci could distinguish three groups of native breeds and their crossbreeds; 11) Awassi, 2) Kivircik-Turkgeldi and 3) Akkaraman-Konya Merino.Öğe Farklı Irktan Koçlarda Kan ve Seminal Plazma Demir, Bakır Düzeyleri ve Düzeyler Arasındaki İlişkiler(2001) Altunok, Vahdettin; Başpınar, NuriBu çalışmada, Konya Hayvancılık Merkez Araştırma Enstitüsünde yetiştirilen beşer baş İvesi, Akkaraman, Merinos ve Koriedale ırkı koçların (sağlıklı, fertil ve 2-3 yaşlı) kan ve seminal plazma demir (Fe) ve bakır (Cu) düzeyleri araştırıldı. Kan ve semen örnekleri Konya ve çevresi için aşım sezonu olan Kasım ayında 2 gün aralıklarla 4 kez alındı ve elde edilen kan ve seminal plazma örneklerinde Fe ve Cu analizleri atomik absorbsiyon spektrofotometresi ile gerçekleştirildi. İvesi, Akkaraman, Merinos, Koriedale ırkı koçlarda kan ve seminal plazma Fe konsantrasyonları sırasıyla : 155.4016.91 \mu g/dl, 141.65l6.02 \mu g/dl, 172.55l5.04 \mu g/dl, 160.25l2.33 \mu g/dl ve 80.202.65 \mu g/dl, 69.105.64 \mu g/dl, 58.853.01 \mu g/dl, 66.405.14 \mu g/dl olarak ölçüldü. Kan ve seminal plazma Cu düzeyleri ise yine aynı sıra ile : 54.755.43 \mu g/dl, 62.506.69 \mu g/dl, 68.009.47 \mu g/dl, 62.453.40 \mu g/dl ve 47.355.55 \mu g/dl, 37.705.33 \mu g/dl, 32.852.82 \mu g/dl, 33.703.05 \mu g/dl olarak belirlendi. Çalışılan koç ırkları arasında seminal plazma Fe konsantrasyonlarının istatistiksel olarak önemli (p0.05) düzeyde farklı olduğu görüldü. Ayrıca seminal plazma Fe ve Cu konsantrasyonları arasında pozitif önemli (p0.01) korrelasyonun varlığı tespit edildi.
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