Detection of Brucella Canis From Inguinal Lymph Nodes of Naturally Infected Dogs by PCR
Yükleniyor...
Dosyalar
Tarih
2010
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Inc
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The aim of this study was to standardize and evaluate a PCR assay for the detection of Brucella canis (B. canis) in lymph node samples of naturally infected dogs. The performance of the PCR was compared with the results of bacteriological culture as reference method. Forty-eight inguinal lymph node samples were collected from 48 dogs (18 males and 30 females) that died in the city's pound in the years 2007-2008 and were examined by microbiological culture and the PCR assay. B. canis was isolated from 4 (8.3%) of 48 lymph node samples. Forty-four (91.7%) of the samples were bacteriological culture negative. B. canis DNA was directly detected from all culture positive lymph node samples (n = 4) by PCR. All of the culture negative samples were confirmed as negative by PCR. When the culture method was used as a gold standard, sensitivity and specificity of the PCR assay were found to be 100%. The limit of PCR detection of B. canis DNA was 1.4 x 101 CFU/g at least. In conclusion, the PCR assay has been shown to have a diagnostic performance equal to bacteriological culture for detection of B. canis. By a non-hazardous protocol for laboratory workers, the assay can be performed in one day.
Açıklama
Anahtar Kelimeler
Canine brucellosis, PCR, Microbiological culture, Lymph node
Kaynak
Theriogenology
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
74
Sayı
Künye
Aras, Z., Uçan, U. S., (2010). Detection of Brucella Canis From Inguinal Lymph Nodes of Naturally Infected Dogs by PCR. Theriogenology, (74), 658-662. Doi: 10.1016/j.theriogenology.2010.03.023
