Detection of Brucella Canis From Inguinal Lymph Nodes of Naturally Infected Dogs by PCR

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dc.contributor.authorAras, Zeki
dc.contributor.authorUçan, Uçkun Sait
dc.date.accessioned2020-03-26T17:47:47Z
dc.date.available2020-03-26T17:47:47Z
dc.date.issued2010
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe aim of this study was to standardize and evaluate a PCR assay for the detection of Brucella canis (B. canis) in lymph node samples of naturally infected dogs. The performance of the PCR was compared with the results of bacteriological culture as reference method. Forty-eight inguinal lymph node samples were collected from 48 dogs (18 males and 30 females) that died in the city's pound in the years 2007-2008 and were examined by microbiological culture and the PCR assay. B. canis was isolated from 4 (8.3%) of 48 lymph node samples. Forty-four (91.7%) of the samples were bacteriological culture negative. B. canis DNA was directly detected from all culture positive lymph node samples (n = 4) by PCR. All of the culture negative samples were confirmed as negative by PCR. When the culture method was used as a gold standard, sensitivity and specificity of the PCR assay were found to be 100%. The limit of PCR detection of B. canis DNA was 1.4 x 101 CFU/g at least. In conclusion, the PCR assay has been shown to have a diagnostic performance equal to bacteriological culture for detection of B. canis. By a non-hazardous protocol for laboratory workers, the assay can be performed in one day.en_US
dc.identifier.citationAras, Z., Uçan, U. S., (2010). Detection of Brucella Canis From Inguinal Lymph Nodes of Naturally Infected Dogs by PCR. Theriogenology, (74), 658-662. Doi: 10.1016/j.theriogenology.2010.03.023
dc.identifier.doi10.1016/j.theriogenology.2010.03.023en_US
dc.identifier.endpage662en_US
dc.identifier.issn0093-691Xen_US
dc.identifier.issn1879-3231en_US
dc.identifier.pmid20452003en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage658en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.theriogenology.2010.03.023
dc.identifier.urihttps://hdl.handle.net/20.500.12395/24765
dc.identifier.volume74en_US
dc.identifier.wosWOS:000281261600022en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorAras, Zeki
dc.institutionauthorUçan, Uçkun Sait
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofTheriogenologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCanine brucellosisen_US
dc.subjectPCRen_US
dc.subjectMicrobiological cultureen_US
dc.subjectLymph nodeen_US
dc.titleDetection of Brucella Canis From Inguinal Lymph Nodes of Naturally Infected Dogs by PCRen_US
dc.typeArticleen_US

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