Evaluation of random amplified polymorphic DNA and multiple-locus variable number tandem repeat analyses for Mycoplasma cynos

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dc.contributor.authorSakmanoglu, Aslı.
dc.contributor.authorSayin, Zafer.
dc.contributor.authorPinarkara, Yasemin.
dc.contributor.authorUslu, Ali.
dc.contributor.authorUcan, Uckun Sait.
dc.contributor.authorErganis, Osman.
dc.date.accessioned2020-03-26T20:14:04Z
dc.date.available2020-03-26T20:14:04Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Temel Bilimler Bölümüen_US
dc.description.abstractMycoplasma spp. can cause diseases of the respiratory system as well as urogenital infections, infertility, and anemia. The members of this genus have a low G + C content compared to other bacteria. Because primers used in the random amplified polymorphic DNA (RAPD) technique are only 10 bp long and have high GC content, this method can be inadequate for genotyping Mycoplasma spp. isolates. The aim of this study was to develop and evaluate multiple-locus variable number tandem repeat analysis (MLVA) and two-primer RAPD (TP-RAPD) procedures for subtyping Mycoplasma cynos isolates. A total of 55 M. cynos isolates obtained from 162 bronchoalveolar lavage fluid samples from shelter and pet dogs were used in this study. Seventy-four tandem repeat regions were detected in the M. cynos genome, and two of these loci were determined to be suitable and used for development of the MLVA scheme. The results of variable number tandem repeat (VNTR) analysis and TP-RAPD-PCR were compared with RAPD-PCR. The discriminatory power of TP-RAPD-PCR (Hunter-Gaston diversity index [HGDI] = 0.84) was higher than those of RAPD-PCR (HGDI = 0.727), VNTR1 (HGDI = 0.8), and VNTR3 (HGDI = 0.757). We observed that the TPRAPD-PCR and MLVA methods provide clearer data and are more successful in determining genetic diversity, in contrast to the RAPD-PCR method for this species.en_US
dc.description.sponsorshipCoordinatorship for Scientific Research Projects of Selcuk University [SUBAPK 17401041]en_US
dc.description.sponsorshipThis study was part of a research project supported by the Coordinatorship for Scientific Research Projects of Selcuk University (SUBAPK 17401041, Konya, Turkey). This study previously presented partly as an oral presentation at the XIII. Veterinary Microbiology Congress (October 2018-Antalya/Turkey).en_US
dc.identifier.citationSakmanoglu, A., Sayin, Z., Pinarkara, Y., Uslu, A., Ucan, U. S., Erganis, O. (2019). Evaluation of Random Amplified Polymorphic DNA and Multiple-Locus Variable Number Tandem Repeat Analyses for Mycoplasma Cynos. Journal of Microbiological Methods, 161, 1-7.
dc.identifier.doi10.1016/j.mimet.2019.04.004en_US
dc.identifier.endpage7en_US
dc.identifier.issn0167-7012en_US
dc.identifier.issn1872-8359en_US
dc.identifier.pmid30981711en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.mimet.2019.04.004
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37815
dc.identifier.volume161en_US
dc.identifier.wosWOS:000470944100001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorSakmanoglu, Aslı.
dc.institutionauthorSayin, Zafer.
dc.institutionauthorPinarkara, Yasemin.
dc.institutionauthorUslu, Ali.
dc.institutionauthorUcan, Uckun Sait.
dc.institutionauthorErganis, Osman.
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofJOURNAL OF MICROBIOLOGICAL METHODSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBronchoalveolar lavage fluiden_US
dc.subjectMLVAen_US
dc.subjectMycoplasma cynosen_US
dc.subjectRAPDen_US
dc.subjectTP-RAPDen_US
dc.titleEvaluation of random amplified polymorphic DNA and multiple-locus variable number tandem repeat analyses for Mycoplasma cynosen_US
dc.typeArticleen_US

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