Nuclear magnetic resonance (NMR)-based metabolome profile evaluation in dairy cows with and without displaced abomasum

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dc.contributor.authorBasoglu, Abdullah.
dc.contributor.authorBaspinar, Nuri.
dc.contributor.authorTenori, Leonardo.
dc.contributor.authorLicari, Cristina.
dc.contributor.authorGulersoy, Erdem.
dc.date.accessioned2020-03-26T20:20:24Z
dc.date.available2020-03-26T20:20:24Z
dc.date.issued2020
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Klinik Bilimler Bölümüen_US
dc.description.abstractBackground: Displaced abomasum (DA) is a condition of dairy cows that severely impacts animal welfare and causes huge economic losses. Objective: To assess the metabolic status of the disease using metabolomics in serum, urine and liver samples aimed at both water soluble and lipid soluble fractions. Methods: Fifty Holstein multiparous cows with DA (42 left, 8 right) and 20 clinically healthy Holstein multiparous cows were used. Left DA was associated with concomitant ketosis in 19 animals and right in two. NMR-based metabolomics approach and hematological and biochemical analyses were performed. Statistical analysis was carried out on H-1-NMR data after they have been normalized using PQN method. Results: Contrary to generated PCA score plots the OPLS-supervised method revealed differences between healthy animals and diseased ones based on serum water-soluble samples. While water and lipid soluble metabolites decreased in serum samples, fatty acid fractions and cholesterol were increased in liver samples in DA affected cows. The metabolomic and chemical profiles clearly revealed that cows with DA (especially with LDA) were at risk of ketosis and fatty liver. Serum hippuric acid concentration was significantly higher in healthy cows in comparison with LDA, whereas serum glycine concentration was reported higher for healthy when compared to RDA affected animals. Conclusion: A biochemical network and pathway mapping revealed 'valine, leucine and isoleucine biosynthesis' and 'phenylalanine, tyrosine and tryptophan biosynthesis' as the most probable altered metabolic pathway in DA condition. Serum was advocated as the optimal biological matrix for the H-1-NMR analysis.en_US
dc.description.sponsorshipSelcuk University Scientific Research Projects Coordination UnitSelcuk University [17102035]; EC Contract INEXT [653706]en_US
dc.description.sponsorshipThis work was financially supported by the Selcuk University Scientific Research Projects Coordination Unit (Project No: 17102035). CERM/CIRMMP center of the ESFRI Instruct is gratefully acknowledged for the NMR access provision financially supported by the EC Contract INEXT No 653706.en_US
dc.identifier.citationBasoglu, A., Baspinar, N., Tenori, L., Licari, C., Gulersoy, E. (2020). Nuclear Magnetic Resonance (NMR)-Based Metabolome Profile Evaluation in Dairy Cows with and without Displaced Abomasum. Veterinary Quarterly, 40(1), 1-15.
dc.identifier.doi10.1080/01652176.2019.1707907en_US
dc.identifier.endpage15en_US
dc.identifier.issn0165-2176en_US
dc.identifier.issn1875-5941en_US
dc.identifier.issue1en_US
dc.identifier.pmid31858882en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://dx.doi.org/10.1080/01652176.2019.1707907
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38577
dc.identifier.volume40en_US
dc.identifier.wosWOS:000506123800001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBasoglu, Abdullah.
dc.institutionauthorBaspinar, Nuri.
dc.institutionauthorGulersoy, Erdem.
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.relation.ispartofVETERINARY QUARTERLYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCattleen_US
dc.subjectcowen_US
dc.subjectmetabolomicsen_US
dc.subjectNMRen_US
dc.subjectdisplaced abomasumen_US
dc.titleNuclear magnetic resonance (NMR)-based metabolome profile evaluation in dairy cows with and without displaced abomasumen_US
dc.typeArticleen_US

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