A novel polymerase chain reaction to detect brucella canis in dogs
Yükleniyor...
Dosyalar
Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
KAFKAS UNIV, VETERINER FAKULTESI DERGISI
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, the specific polymerase chain reaction has been standardized and evaluated for the direct diagnosis of Brucella canis in vaginal swab samples from dogs. The specific primer sets are directed to the 16S-23S rRNA inter-space region of Brucella spp. and the deletion of 351 bp in BMEI1426-BMEI1427 in B. canis. A total of 21 references and field strains and 35 vaginal swab samples were used for the evaluation of the polymerase chain reaction. It found that polymerase chain reaction is positive for B. canis DNA indicated by only amplification of 214 bp product. It detected at least 2.7 x 10(1) CFU/g of bacteria diluted in vaginal swab samples indicates that the polymerase chain reaction can be used as a practical alternative for bacterial isolation. The novel polymerase chain reaction provides a simple and rapid for the detection of B. canis in clinical and field samples in one step and in short time about 24 h.
Açıklama
Anahtar Kelimeler
Specific PCR, Brucella canis, Vaginal swab samples
Kaynak
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
21
Sayı
2
Künye
Aras, Z., Taşpınar, M., Aydın, İ. (2015). A Novel Polymerase Chain Reaction to Detect Brucella Canis in Dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21(2), 169-172.
