A novel polymerase chain reaction to detect brucella canis in dogs

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dc.contributor.authorAras, Zeki
dc.contributor.authorTaspinar, Mehmet
dc.contributor.authorAydin, Ibrahim
dc.date.accessioned2020-03-26T19:00:31Z
dc.date.available2020-03-26T19:00:31Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Klinik Bilimler Bölümüen_US
dc.description.abstractIn this study, the specific polymerase chain reaction has been standardized and evaluated for the direct diagnosis of Brucella canis in vaginal swab samples from dogs. The specific primer sets are directed to the 16S-23S rRNA inter-space region of Brucella spp. and the deletion of 351 bp in BMEI1426-BMEI1427 in B. canis. A total of 21 references and field strains and 35 vaginal swab samples were used for the evaluation of the polymerase chain reaction. It found that polymerase chain reaction is positive for B. canis DNA indicated by only amplification of 214 bp product. It detected at least 2.7 x 10(1) CFU/g of bacteria diluted in vaginal swab samples indicates that the polymerase chain reaction can be used as a practical alternative for bacterial isolation. The novel polymerase chain reaction provides a simple and rapid for the detection of B. canis in clinical and field samples in one step and in short time about 24 h.en_US
dc.identifier.citationAras, Z., Taşpınar, M., Aydın, İ. (2015). A Novel Polymerase Chain Reaction to Detect Brucella Canis in Dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21(2), 169-172.
dc.identifier.doi10.9775/kvfd.2014.11977en_US
dc.identifier.endpage172en_US
dc.identifier.issn1300-6045en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage169en_US
dc.identifier.urihttps://dx.doi.org/10.9775/kvfd.2014.11977
dc.identifier.urihttps://hdl.handle.net/20.500.12395/31786
dc.identifier.volume21en_US
dc.identifier.wosWOS:000349190400004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorAydin, Ibrahim
dc.language.isoenen_US
dc.publisherKAFKAS UNIV, VETERINER FAKULTESI DERGISIen_US
dc.relation.ispartofKAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectSpecific PCRen_US
dc.subjectBrucella canisen_US
dc.subjectVaginal swab samplesen_US
dc.titleA novel polymerase chain reaction to detect brucella canis in dogsen_US
dc.typeArticleen_US

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