Uncovering sperm metabolome to discover biomarkers for bull fertility

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dc.authorid0000-0002-8335-5645
dc.contributor.authorMenezes, E. B.
dc.contributor.authorVelho, A. L. C.
dc.contributor.authorSantos, F.
dc.contributor.authorDinh, T.
dc.contributor.authorKaya, A.
dc.contributor.authorTopper, E.
dc.contributor.authorMoura, A. A.
dc.contributor.authorMemili, E.
dc.date.accessioned2020-03-26T20:19:41Z
dc.date.available2020-03-26T20:19:41Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Klinik Bilimler Bölümüen_US
dc.description.abstractBackground Subfertility decreases the efficiency of the cattle industry because artificial insemination employs spermatozoa from a single bull to inseminate thousands of cows. Variation in bull fertility has been demonstrated even among those animals exhibiting normal sperm numbers, motility, and morphology. Despite advances in research, molecular and cellular mechanisms underlying the causes of low fertility in some bulls have not been fully elucidated. In this study, we investigated the metabolic profile of bull spermatozoa using non-targeted metabolomics. Statistical analysis and bioinformatic tools were employed to evaluate the metabolic profiles high and low fertility groups. Metabolic pathways associated with the sperm metabolome were also reported. Results A total of 22 distinct metabolites were detected in spermatozoa from bulls with high fertility (HF) or low fertility (LF) phenotype. The major metabolite classes of bovine sperm were organic acids/derivatives and fatty acids/conjugates. We demonstrated that the abundance ratios of five sperm metabolites were statistically different between HF and LF groups including gamma-aminobutyric acid (GABA), carbamate, benzoic acid, lactic acid, and palmitic acid. Metabolites with different abundances in HF and LF bulls had also VIP scores of greater than 1.5 and AUC- ROC curves of more than 80%. In addition, four metabolic pathways associated with differential metabolites namely alanine, aspartate and glutamate metabolism, beta-alanine metabolism, glycolysis or gluconeogenesis, and pyruvate metabolism were also explored. Conclusions This is the first study aimed at ascertaining the metabolome of spermatozoa from bulls with different fertility phenotype using gas chromatography-mass spectrometry. We identified five metabolites in the two groups of sires and such molecules can be used, in the future, as key indicators of bull fertility.en_US
dc.description.sponsorshipAgriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2017-67016-26507]; Mississippi Agricultural Experiment Station; Alta Genetics Inc.; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) of BrazilNational Council for Scientific and Technological Development (CNPq); CNPqNational Council for Scientific and Technological Development (CNPq); Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) of BrazilCAPESen_US
dc.description.sponsorshipThis project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2017-67016-26507 from the USDA National Institute of Food and Agriculture. Partial funding was also provided by Mississippi Agricultural Experiment Station, Alta Genetics Inc., and by Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) of Brazil. F. Santos was funded by competitive fellowships from CNPq, and both A. Velho and E. Menezes were funded by Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) of Brazil. The funding agencies of this study had no role in the design of the study, data collection and analysis, interpretation of data, the decision to submit the report for publication or preparation of the manuscript.en_US
dc.identifier.citationMenezes, E. B., Velho, A. L. C., Santos, F., Dinh, T., Kaya, A., Topper, E., Moura, A. A., Memili, E. (2019). Uncovering Sperm Metabolome to Discover Biomarkers for Bull Fertility. BMC Genomics, 20(1), 1-16
dc.identifier.doi10.1186/s12864-019-6074-6en_US
dc.identifier.endpage16
dc.identifier.issn1471-2164en_US
dc.identifier.issue1en_US
dc.identifier.pmid31533629en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1
dc.identifier.urihttps://dx.doi.org/10.1186/s12864-019-6074-6
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38367
dc.identifier.volume20en_US
dc.identifier.wosWOS:000486997200001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaya, A.
dc.language.isoenen_US
dc.publisherBMCen_US
dc.relation.ispartofBMC GENOMICSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectMetabolomicsen_US
dc.subjectBovineen_US
dc.subjectSpermatozoaen_US
dc.subjectGas chromatographyen_US
dc.subjectMass spectrometryen_US
dc.titleUncovering sperm metabolome to discover biomarkers for bull fertilityen_US
dc.typeArticleen_US

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