Metabolite profiling by hyphenated liquid chromatographic mass spectrometric technique (hplc-dad-esı-q-tof-ms/ms) and neurobiological potential of haplophyllum sahinii and h. vulcanicum extracts

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dc.contributor.authorKarahisar, Esengul
dc.contributor.authorTugay, Osman
dc.contributor.authorOrhan, Ilkay Erdogan
dc.contributor.authorDeniz, Fatma Sezer Senol
dc.contributor.authorLuca, Simon Vlad
dc.contributor.authorSkalicka-Wozniak, Krystyna
dc.contributor.authorSahin, Mustafa
dc.date.accessioned2020-03-26T20:15:10Z
dc.date.available2020-03-26T20:15:10Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümüen_US
dc.description.abstractIn the current study, the ethanol extracts of flower, stem, and root parts of two endemic Turkish species, e. g., Haplophyllum sahinii O. Tugay & D. Ulukus and H. vulcanicum Boiss. & Heldr., were screened against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) associated with Alzheimer's disease as well as tyrosinase (TYR) linked to Parkinson's disease using ELISA microplate assay at 200 mu g/mL. Among the extracts, the highest inhibition was caused by the stem extract of H. sahinii against BChE (IC50=64.93 +/- 1.38 mu g/mL). Consistently, all of the extracts were found to exert a selective inhibition towards BChE to some extent. It was only the root extract of H. vulcanicum that could inhibit AChE at low level (IC50=203.18 +/- 5.33 mu g/mL). None of the extracts displayed an inhibition over 50 % against TYR. Metabolite profiling of the extracts was achieved by a highly hyphenated liquid chromatographic mass spectrometric technique (HPLC-DAD-ESI-Q-TOF-MS/MS), which revealed the presence of furoquinoline (beta-fagarine, gamma-fagarine) and amide (tubasenicine, tubacetine) alkaloids; furano- (rutamarin), pyrano- (xanthyletine), and geranyloxy coumarins; phenylpropanoid (secoisolariciresinol), arylnaphthalene (mono-O-acetyldiphyllin apioside), and dibenzylbutyrolactone (kusunokinin, haplomyrfolin) lignans. Several important differences were observed between the extracts analyzed. beta-Fagarine was the major alkaloid in H. vulcanicum, whereas gamma-fagarine was present only in the roots of both Haplophyllum species; moreover, secoisolariciresinol and secoisolariciresinol dimethyl ether were the main lignans in the stems and flowers. This is the first study identifying ChE and TYR inhibitory effect and metabolic profiles of H. vulcanicum and H. sahinii.en_US
dc.identifier.citationKarahisar, E., Tugay, O., Orhan, I. E., Sezer Senol Deniz, F., Vlad Luca, S., Skalicka‐Wozniak, K., Sahin, M. (2019). Metabolite Profiling By Hyphenated Liquid Chromatographic Mass Spectrometric Technique (HPLC‐DAD‐ESI‐Q‐TOF‐MS/MS) And Neurobiological Potential Of Haplophyllum Sahinii And H. Vulcanicum Extracts. Chemistry & Biodiversity, 16,(9), e1900333.
dc.identifier.doi10.1002/cbdv.201900333en_US
dc.identifier.issn1612-1872en_US
dc.identifier.issn1612-1880en_US
dc.identifier.issue9en_US
dc.identifier.pmid31365785en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://dx.doi.org/10.1002/cbdv.201900333
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37994
dc.identifier.volume16en_US
dc.identifier.wosWOS:000480882800001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKarahisar, Esengul
dc.institutionauthorTugay, Osman
dc.institutionauthorSahin, Mustafa
dc.language.isoenen_US
dc.publisherWILEY-V C H VERLAG GMBHen_US
dc.relation.ispartofCHEMISTRY & BIODIVERSITYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectHaplophyllum sahiniien_US
dc.subjectHaplophyllum vulcanicumen_US
dc.subjectcholinesteraseen_US
dc.subjecttyrosinaseen_US
dc.subjectmetabolite profilingen_US
dc.subjectbiological activityen_US
dc.titleMetabolite profiling by hyphenated liquid chromatographic mass spectrometric technique (hplc-dad-esı-q-tof-ms/ms) and neurobiological potential of haplophyllum sahinii and h. vulcanicum extractsen_US
dc.typeArticleen_US

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