Comparison of Antibacterial Activity of Two Dentin Bonding Systems Using Agar Well Technique and Tooth Cavity Model

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dc.contributor.authorÖzer, F.
dc.contributor.authorKarakaya, S.
dc.contributor.authorÜnlü, Nimet
dc.contributor.authorErganiş, Osman
dc.contributor.authorKav, K.
dc.contributor.authorImazato, S.
dc.date.accessioned2020-03-26T16:45:30Z
dc.date.available2020-03-26T16:45:30Z
dc.date.issued2003
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractObjective. This study compared the antibacterial activities of two dentin bonding systems (ABF, Kuraray and Reactmer Bond, Shofu) by a conventional agar well technique and a newly designed in vitro test using tooth model. Methods. In the agar well technique, the test materials were filled in the welts of Muller Hinton agar plates inoculated with Streptococcus mutans NCTC10449, and the diameters of inhibition zones produced around the materials were measured after 24 h of incubation. For the tooth model test, three cavities (diameter 1 mm, depth 2 mm) were prepared in the flat occlusal dentin of human extracted molar. After sterilization, the teeth were left in broth culture of 1.56 x 10(8) CFU/ml of S. mutans at 37 degreesC for 72 h for allowing bacteria to invade the cavity. The dentin bonding systems were applied separately to each of the two infected cavities, and the third cavity was left unapplied for control. After seating the occlusat surfaces, the teeth were kept in physiologic saline solution at 37 degreesC for 72 h. The standardized amounts of dentin chips (120 +/- 5 mg) were obtained from the cavity walls and the number of bacteria recovered was determined. The results were analyzed by One Way ANOVA, Kruskal Wallis and Mann-Whitney's U tests. Results. The primer of ABF and Reactmer Bond produced inhibition zones with similar sizes (p > 0.05), but the bonding resin of ABF did not produce any inhibition. When tested by the mode( cavity method, the application of ABF resulted in significantly less bacterial. recovery than Reactmer Bond (p < 0.05), demonstrating substantial antibacterial effects. Conclusions. The tooth model method used in this study was effective for evaluating the substantial, antibacterial effects of dentin bonding agents, and the experimental dentin bonding system ABF was demonstrated to be able to inactivate the bacteria in the cavity effectively in comparison with little antibacterial activity shown by Reactmer Bond.en_US
dc.identifier.citationÖzer, F., Karakaya, S., Ünlü, N., Erganiş, O., Kav, K., Imazato, S., (2003). Comparison of Antibacterial Activity of Two Dentin Bonding Systems Using Agar Well Technique and Tooth Cavity Model. Journal of Dentistry, (31), 111-116. Doi: 10.1016/S0300-5712(03)00006-X
dc.identifier.doi10.1016/S0300-5712(03)00006-Xen_US
dc.identifier.endpage116en_US
dc.identifier.issn0300-5712en_US
dc.identifier.pmid12654550en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage111en_US
dc.identifier.urihttps://dx.doi.org/10.1016/S0300-5712(03)00006-X
dc.identifier.urihttps://hdl.handle.net/20.500.12395/18397
dc.identifier.volume31en_US
dc.identifier.wosWOS:000181865700004en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorÖzer, F.
dc.institutionauthorKarakaya, S.
dc.institutionauthorÜnlü, Nimet
dc.institutionauthorErganiş, Osman
dc.institutionauthorKav, K.
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofJournal of Dentistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAntibacterial Testsen_US
dc.subjectAntibacterial Activitiesen_US
dc.subjectStreptococcus Mutansen_US
dc.subjectDentin Bonding Systemsen_US
dc.subjectDentin Primersen_US
dc.titleComparison of Antibacterial Activity of Two Dentin Bonding Systems Using Agar Well Technique and Tooth Cavity Modelen_US
dc.typeArticleen_US

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