Embryotoxicity Assay of Aflatoxin Produced by Aspergillus Parasiticus NRRL 2999
Yükleniyor...
Dosyalar
Tarih
2000
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
1. The embryotoxicity of mixed aflatoxins (AF) and aflatoxin B-1 (AFB(1)) were evaluated by a modified chick embryotoxicity screening test (CHEST). Adverse effects on the early embryonic development of thymus and bursa of Fabricius were also investigated by light microscopy. AF consisted of 83.06% AFB(1), 12.98% AFB(2), 2.84% AFG(1) and 1.12% AFG(2). 2. A total of 448 fertilised laying hens' eggs were used. AF and AFB(1) were injected into the eggs at doses of 10, 100 and 1000 ng/egg. Embryonic developmental stages were evaluated according to the Hamburger-Hamilton scale (HH-scale). 3. The results showed that AFB(1) given at 10 ng/egg had a significantly (P<0.05) greater embryotoxic effect than AF given at a similar dose. The higher doses of both AF and AFB(1) caused higher embryonic mortality and also an increase in early deaths. 4. In the groups receiving 100 ng/egg AF and AFB(1) an abnormal development was seen, with a protruded central region, corresponding to the area pellucida of the blastoderm. No other developmental abnormality attributable to AF or AFB(1) was found.
Açıklama
Anahtar Kelimeler
Kaynak
British Poultry Science
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
41
Sayı
4
Künye
Çelik, İ., Oğuz, H., Demet, O., Boydak, M., Dönmez, H. H., Sur, E., Nizamlıoğlu, F., (2000). Embryotoxicity Assay of Aflatoxin Produced by Aspergillus Parasiticus NRRL 2999. British Poultry Science, 41(4), 401-409. Doi: 10.1080/713654961
