Influence of Methionine and Dithioerythritol on Sperm Motility, Lipid Peroxidation and Antioxidant Capacities During Liquid Storage of Ram Semen

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dc.contributor.authorÇoyan, Kenan
dc.contributor.authorBaşpınar, Nuri
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorAkalın, Pınar Peker
dc.contributor.authorAtaman, Mehmet Bozkurt
dc.contributor.authorÖmür, Ali Doğan
dc.contributor.authorGüngör, Şükrü
dc.contributor.authorKüçükgünay, Sadık
dc.contributor.authorÖzkalp, Birol
dc.contributor.authorSarıözkan, Serpil
dc.date.accessioned2020-03-26T17:49:11Z
dc.date.available2020-03-26T17:49:11Z
dc.date.issued2010
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe aim of this study was to investigate the effects of methionine and dithioerythritol, added to the Tris extender, on ram sperm motility and LPO (lipid peroxidation) and antioxidant capacities during liquid storage up to 72 h at 5 degrees C. Ejaculates collected from five Merino rams, were evaluated and pooled at 37 degrees C. This study included two experiments. In experiment 1, each pooled ejaculate was divided into four equal aliquots and diluted (37 degrees C) with the base extender, containing 0 (control), 1, 2 and 4 mM methionine, at a final concentration of approximately 4 x 10(8) sperms/ml (single step dilution), in a 15-ml plastic centrifuge tube. In experiment 2, dithioerythritol, at concentrations of 0 (control). 0.5, 1 and 2 mM, was used as an additive in the extender, and the procedure explained above was applied for the division of aliquots and the dilution of semen. Diluted semen samples were kept in glass tubes and cooled from 37 to 5 degrees C in a cold cabinet, and maintained at 5 degrees C. Sperm motility and LPO and total glutathione (GSH) and glutathione peroxidase (GPx) capacities were determined at 5 degrees C for periods of 0, 24,48 and 72 h of liquid storage. The extender supplemented with 1 mM methionine led to higher motility percentages (77.0 +/- 1.2%), in comparison to the control group (66.0 +/- 4.9%), during 72 h of liquid storage (P < 0.05). As regards dithioerythritol, it did not statistically improve the motility rates for any of the storage times at 5 degrees C. In biochemical assays, differences in LPO levels between the groups with antioxidants and the control groups were not statistically significant. Compared to the control group, no significant difference was observed in GSH and GPx activities following the addition of methionine, during 72 h of storage. Total GSH and GPx activities did not increase significantly upon supplementation with 0.5 and 1 mM of dithioerythritol, compared to the control group, at any of the time points (P > 0.05). Dithioerythritol at 2 mM led (P < 0.01) to elevating GSH activity, compared to the control group, during 72 h of liquid storage. GPx activity was approximately 10 times higher for 2 mM of dithioerythritol (P < 0.001), compared to that of the control group at all time points. The question regarding the sustainability of sperm survival, LPO and antioxidant capacities following liquid storage of semen remains unanswered. Further studies are required for a better understanding of the biochemical changes and to obtain more information on the determination of lipid peroxidation and antioxidant capacities during cooled storage of ram semen.en_US
dc.description.sponsorshipSelcuk UniversitySelcuk University; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [108 O 522]en_US
dc.description.sponsorshipThis study was financed under the project supported by Selcuk University, Directorate of Scientific Research Projects (BAP) and by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project No: 108 O 522).en_US
dc.identifier.citationÇoyan, K., Başpınar, N., Bucak, M. N., Akalın, P. P., Ataman, M. B., Ömür, A. D., Güngör, Ş., Küçükgünay, S., Özkalp, B., Sarıözkan, S., (2010). Influence of Methionine and Dithioerythritol on Sperm Motility, Lipid Peroxidation and Antioxidant Capacities During Liquid Storage of Ram Semen. Research in Veterinary Science, (89), 426-431. Doi: 10.1016/j.rvsc.2010.03.025
dc.identifier.doi10.1016/j.rvsc.2010.03.025en_US
dc.identifier.endpage431en_US
dc.identifier.issn0034-5288en_US
dc.identifier.issn1532-2661en_US
dc.identifier.pmid20403626en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage426en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.rvsc.2010.03.025
dc.identifier.urihttps://hdl.handle.net/20.500.12395/25001
dc.identifier.volume89en_US
dc.identifier.wosWOS:000284664000018en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorÇoyan, Kenan
dc.institutionauthorBaşpınar, Nuri
dc.institutionauthorAtaman, Mehmet Bozkurt
dc.institutionauthorÖmür, Ali Doğan
dc.institutionauthorGüngör, Şükrü
dc.institutionauthorÖzkalp, Birol
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofResearch in Veterinary Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectRam semenen_US
dc.subjectMethionineen_US
dc.subjectDithioerythritolen_US
dc.subjectLiquid storageen_US
dc.subjectLipid peroxidationen_US
dc.subjectAntioxidant capacityen_US
dc.titleInfluence of Methionine and Dithioerythritol on Sperm Motility, Lipid Peroxidation and Antioxidant Capacities During Liquid Storage of Ram Semenen_US
dc.typeArticleen_US

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