Species-Specificity of Growth-Promoting Effects of Prolactin During Rat Embryogenesis
Yükleniyor...
Dosyalar
Tarih
1998
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
WILEY
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In the early stages of embryonic development, many growth-promoting molecules must be provided by the maternal system. The molecules involved in growth processes may be either hormones or growth factors, or molecules that interact with such factors. The pregnancy related hormone, prolactin (PRL, MW 23 kDa) has been implicated in the control of embryonic growth. The growth-promoting potential of PRL and its species-specificity was investigated by culturing 9.5 d rat embryos in vitro for 48 h in depleted serum in the presence and absence of PRL from 3 different species. The growth-supporting capacity of the serum was reduced by removal of low molecular weight molecules by prolonged filtration of the serum using filters with a molecular weight exclusion of 30 kDa. This method provided a 'semidefined' medium (retenate) in which embryonic growth and development was significantly reduced, demonstrating that the low molecular weight fraction of serum may contain some growth-promoting factors. Addition of PRL (0.4-25.6 ng/ml) from different species (human, sheep and rat) to retenate significantly improved embryonic growth and development, suggesting that the developing embryo may utilise PRL. Amongst PRLs, rat PRL was found to be active at much lower concentrations than either of the other molecules, and human PRL had more effect in low concentrations than sheep PRL suggesting a species-specificity for this hormone. It may be that the PRL receptors of the rat embryos have greater affinity for the rat hormone as different responses for hormones from different species have been shown. These findings suggest that embryos may be able to utilise maternally derived PRL during organogenesis.
Açıklama
Anahtar Kelimeler
Whole Embryo Culture, Rat, Organogenesis, Development
Kaynak
Journal of Anatomy
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
192
Sayı
Künye
Karabulut, A. K., Pratten, M. K., (1998). Species-Specificity of Growth-Promoting Effects of Prolactin During Rat Embryogenesis. Journal of Anatomy, 192(1998), 1-12. Doi:10.1046/j.1469-7580.1998.19210001.x
