Teratogenicity of Edoferon Kappa A, a Molecule Derived From Salicylate, in Cultured Rat Embryos: Differences From Salicylate and Interaction With Free Oxygen Radical Scavenging Enzymes
Yükleniyor...
Dosyalar
Tarih
2000
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wıley
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The effect of edoferon kappa A (E-KA), a non-specific immunomodulatory and anti-neoplastic chemical substance derived from the methyl form of salicylate (acetyl salicylic acid; ASA), on mammalian embryos was studied and compared to the effects of ASA. Rat embryos were cultured in vitro from 9.5 days of gestation for 48 h. E-KA (0.1-12.8 mg/ml) and ASA (0.1-0.6 mg/ml) were added to the whole rat serum. To investigate the interaction of these molecules with antioxidant agents, the lowest effective concentrations of E-KA (0.6 mg/ml) and ASA (0.3 mg/ml) for all parameters were added to the culture media in the presence of superoxide dismutase (SOD) (30 U/ ml) or glutathione (0.5 mu mol/ml). The growth and development of embryos was compared and each embryo was evaluated for the presence of any malformations. E-KA and ASA decreased growth and development in a concentration-responsive manner. There was also a concentration-related increase in overall dysmorphology (haematoma in the yolk sac and neural system, open neural tube, abnormal tail torsion and the absence of fore limb bud). There were no statistically significant differences between the control and embryos grown in the presence of 0.1-0.4 mg/ml E-KA, although the effects of ASA started at a concentration of 0.2 mg/ml. Embryos showed significant growth retardation in all scoring criteria and severe malformations when 0.5-3.2 mg/ml E-KA and 0.3-0.6 mg/ml ASA were added. When SOD was added, there was a significant decrease in the incidence of malformations and growth and developmental parameters were increased but this decrease never reached the control level. We concluded that E-KA has direct toxic effects on the developing embryo but at much higher concentrations than ASA, and the teratogenic effects of these molecules might be related to free oxygen radicals.
Açıklama
Anahtar Kelimeler
Kaynak
Anatomia Histologia Embryologia
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
29
Sayı
6
Künye
Karabulut, A. K., Ülger, H., Pratten, M., (2000). Teratogenicity of Edoferon Kappa A, a Molecule Derived From Salicylate, in Cultured Rat Embryos: Differences From Salicylate and Interaction With Free Oxygen Radical Scavenging Enzymes. Anatomia Histologia Embryologia, 29(6), 363-370. Doi. 10.1046/j.1439-0264.2000.00287.x
