Teratogenicity of Edoferon Kappa A, a Molecule Derived From Salicylate, in Cultured Rat Embryos: Differences From Salicylate and Interaction With Free Oxygen Radical Scavenging Enzymes

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dc.contributor.authorKarabulut, A. K.
dc.contributor.authorÜlger, H.
dc.contributor.authorPratten, M.
dc.date.accessioned2020-03-26T16:36:20Z
dc.date.available2020-03-26T16:36:20Z
dc.date.issued2000
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe effect of edoferon kappa A (E-KA), a non-specific immunomodulatory and anti-neoplastic chemical substance derived from the methyl form of salicylate (acetyl salicylic acid; ASA), on mammalian embryos was studied and compared to the effects of ASA. Rat embryos were cultured in vitro from 9.5 days of gestation for 48 h. E-KA (0.1-12.8 mg/ml) and ASA (0.1-0.6 mg/ml) were added to the whole rat serum. To investigate the interaction of these molecules with antioxidant agents, the lowest effective concentrations of E-KA (0.6 mg/ml) and ASA (0.3 mg/ml) for all parameters were added to the culture media in the presence of superoxide dismutase (SOD) (30 U/ ml) or glutathione (0.5 mu mol/ml). The growth and development of embryos was compared and each embryo was evaluated for the presence of any malformations. E-KA and ASA decreased growth and development in a concentration-responsive manner. There was also a concentration-related increase in overall dysmorphology (haematoma in the yolk sac and neural system, open neural tube, abnormal tail torsion and the absence of fore limb bud). There were no statistically significant differences between the control and embryos grown in the presence of 0.1-0.4 mg/ml E-KA, although the effects of ASA started at a concentration of 0.2 mg/ml. Embryos showed significant growth retardation in all scoring criteria and severe malformations when 0.5-3.2 mg/ml E-KA and 0.3-0.6 mg/ml ASA were added. When SOD was added, there was a significant decrease in the incidence of malformations and growth and developmental parameters were increased but this decrease never reached the control level. We concluded that E-KA has direct toxic effects on the developing embryo but at much higher concentrations than ASA, and the teratogenic effects of these molecules might be related to free oxygen radicals.en_US
dc.identifier.citationKarabulut, A. K., Ülger, H., Pratten, M., (2000). Teratogenicity of Edoferon Kappa A, a Molecule Derived From Salicylate, in Cultured Rat Embryos: Differences From Salicylate and Interaction With Free Oxygen Radical Scavenging Enzymes. Anatomia Histologia Embryologia, 29(6), 363-370. Doi. 10.1046/j.1439-0264.2000.00287.x
dc.identifier.doi10.1046/j.1439-0264.2000.00287.xen_US
dc.identifier.endpage370en_US
dc.identifier.issn0340-2096en_US
dc.identifier.issn1439-0264en_US
dc.identifier.issue6en_US
dc.identifier.pmid11199481en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage363en_US
dc.identifier.urihttps://dx.doi.org/10.1046/j.1439-0264.2000.00287.x
dc.identifier.urihttps://hdl.handle.net/20.500.12395/17255
dc.identifier.volume29en_US
dc.identifier.wosWOS:000166022100007en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKarabulut, A. K.
dc.language.isoenen_US
dc.publisherWıleyen_US
dc.relation.ispartofAnatomia Histologia Embryologiaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleTeratogenicity of Edoferon Kappa A, a Molecule Derived From Salicylate, in Cultured Rat Embryos: Differences From Salicylate and Interaction With Free Oxygen Radical Scavenging Enzymesen_US
dc.typeArticleen_US

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