Cytotoxic Effects of Dental Desensitizers on Human Gingival Fibroblasts

Basit Öğe Kaydı
dc.contributor.authorŞengün, A.
dc.contributor.authorBüyükbaş, S.
dc.contributor.authorHakkı, S. S.
dc.date.accessioned2020-03-26T17:03:18Z
dc.date.available2020-03-26T17:03:18Z
dc.date.issued2006
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe purpose of this study was to evaluate the effects of three different desensitizers on the cell viability and morphology of human gingival fibroblasts (HGF). Human gingival tissues were obtained from individuals who have clinically, healthy periodontium. HGF were grown at 37 degrees C in humidified atmosphere of 5% CO2 in Dulbecco's modified eagle's medium, supplemented with glutamine, penicillin, streptomycin, and 10% fetal bovine serum. The cells were treated with different concentrations (0.1, 0.3, and 0.5 mu L/mL) of desensitizers (Gluma Desensitizer, Seal&Protect, and MicroPrime). After 24- and 48-h exposure to the desensitizer solutions, the viable cells were examined using a hemocytometer. To monitor HGF viability, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay was used and cell morphology was also observed at 48 h. Following exposure to concentrations of 0.1 mu L/mL of test materials for 24 h, cell survival rates for Gluma Desensitizer (106%) and Micro Prime (62%) were not significantly different from the control, while it was significant for Seal&Protect (50%). Growing cells were significantly inhibited by all tested materials for 48 h (p < 0.05) in survival rates of 51, 47, and 31%, respectively. On the basis of the MTT assay, the cytotoxic effect of MicroPrime was more prominent, especially at high concentrations, than does Gluma Desensitizer and Seal&Protect. After exposure to Seal&Protect and MicroPrime, HGF became retracted, rounded in appearance and had loss of normal organization, leading to enlargement of intercellular space when compared with Gluma Desensitizer. As a conclusion, taking the limitations of an in vitro experiment into consideration, the cytotoxic effects were varied, depending on the chemical composition and exposure periods of the tested desensitizers.en_US
dc.identifier.citationŞengün, A., Büyükbaş, S., Hakkı, S. S. (2006). Cytotoxic Effects of Dental Desensitizers on Human Gingival Fibroblasts. Journal of Biomedical Materials Research Part B-applied Biomaterials, (78B), 131-137. Doi: 10.1002/jbm.b.30464
dc.identifier.doi10.1002/jbm.b.30464en_US
dc.identifier.endpage137en_US
dc.identifier.issn1552-4973en_US
dc.identifier.pmid16470823en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage131en_US
dc.identifier.urihttps://dx.doi.org/10.1002/jbm.b.30464
dc.identifier.urihttps://hdl.handle.net/20.500.12395/20417
dc.identifier.volume78Ben_US
dc.identifier.wosWOS:000238737300018en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorŞengün, A.
dc.institutionauthorBüyükbaş, S.
dc.institutionauthorHakkı, S. S.
dc.language.isoenen_US
dc.publisherWiley-Lissen_US
dc.relation.ispartofJournal of Biomedical Materials Research Part B-applied Biomaterialsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectcytotoxicityen_US
dc.subjectMTTen_US
dc.subjectdentalen_US
dc.subjectdesensitizeren_US
dc.subjectfibroblasten_US
dc.titleCytotoxic Effects of Dental Desensitizers on Human Gingival Fibroblastsen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
20417.pdf
Boyut:
299.91 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Makale Dosyası
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Peridontoloji/Makale Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Restoratif Diş Tedavisi/Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Tıbbi Biyokimya/Makale Koleksiyonu