Effects of Antioxidants on Post-thawed Bovine Sperm and Oxidative Stress Parameters: Antioxidants Protect DNA Integrity Against Cryodamage

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dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorTuncer, Purhan Barbaros
dc.contributor.authorSarıözkan, Serpil
dc.contributor.authorBaşpınar, Nuri
dc.contributor.authorTaşpınar, Mehmet
dc.contributor.authorÇoyan, Kenan
dc.contributor.authorBilgili, Ali
dc.contributor.authorAkalın, Pınar Peker
dc.contributor.authorBüyükleblebici, Serhat
dc.contributor.authorAydos, Sena
dc.contributor.authorIlgaz, Seda
dc.contributor.authorSunguroğlu, Asuman
dc.contributor.authorÖztuna, Derya
dc.date.accessioned2020-03-26T17:48:06Z
dc.date.available2020-03-26T17:48:06Z
dc.date.issued2010
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThis study was conducted to determine the effects of methionine, inositol and carnitine on sperm (motility, abnormality, DNA integrity and in vivo fertility) and oxidative stress parameters (lipid peroxidation, total glutathione and antioxidant potential levels) of bovine semen after the freeze-thawing process. Nine ejaculates, collected with the aid of an artificial vagina twice a week from each Simmental bovine, were included in the study. Each ejaculate, splitted into seven equal groups and diluted in Tris-based extender containing methionine (2.5 and 7.5 mM), carnitine (2.5 and 7.5 mM), inositol (2.5 and 7.5 mM) and no additive (control), was cooled to 5 degrees C and then frozen in 0.25 ml straws. Frozen straws were then thawed individually at 37 degrees C for 20 s in a water bath for the evaluation. The extender supplemented with 7.5 mM doses of carnitine and inositol led to higher subjective motility percentages (61.9 +/- 1.3% and 51.3 +/- 1.6%) compared to the other groups. The addition of methionine and carnitine at doses of 2.5 and 7.5 mM and inositol at doses of 7.5 mM provided a greater protective effect in the percentages of total abnormality in comparison to the control and inositol 2.5 mM (P < 0.001). As regards CASA motility, 7.5 mM carnitine (41.6 +/- 2.9% and 54.2 +/- 4.9%) and inositol (34.9 +/- 2.0% and 47.3 +/- 2.2%) caused insignificant increases in CASA and total motility in comparison to the other groups. All of the antioxidants at 2.5 and 7.5 mM resulted in lower sperm with damaged DNA than that of control, thus reducing the DNA damage (P < 0.05). No significant differences were observed in CASA progressive motility and sperm motion characteristics among the groups. In fertility results based on 59-day non-returns, no significant differences were observed in non-return rates among groups. As regards biochemical parameters, supplementation with antioxidants did not significantly affect LPO and total GSH levels in comparison to the control group (P > 0.05). The maintenance of AOP level in methionine 2.5 mM was demonstrated to be higher (5.06 +/- 0.38 mM) than that of control (0.96 +/- 0.29 mM) following the freeze-thawing (P < 0.001). Supplementation with these antioxidants prior to the cryopreservation process protected the DNA integrity against the cryodamage. Furthermore, future research should focus on the molecular mechanisms of the antioxidative effects of the antioxidants methionine, carnitine and inositol during cryopreservation.en_US
dc.description.sponsorshipDirectorate of Lalahan Livestock Central Research Institute, Ankara, TurkeyGida Tarim Ve Hayvancilik Bakanligien_US
dc.description.sponsorshipThis study was supported by Directorate of Lalahan Livestock Central Research Institute, Ankara, Turkey.en_US
dc.identifier.citationBucak, M. N., Tuncer, P. B., Sarıözkan, S., Başpınar, N., Taşpınar, M., Çoyan, K., Bilgili, A., Akalın, P. P., Büyükleblebici, S., Aydos, S., Ilgaz, S., Sunguroğlu, A., Öztuna, D., (2010). Effects of Antioxidants on Post-thawed Bovine Sperm and Oxidative Stress Parameters: Antioxidants Protect DNA Integrity Against Cryodamage. Cryobiology, (61), 248-253. Doi: 10.1016/j.cryobiol.2010.09.001
dc.identifier.doi10.1016/j.cryobiol.2010.09.001en_US
dc.identifier.endpage253en_US
dc.identifier.issn0011-2240en_US
dc.identifier.issn1090-2392en_US
dc.identifier.pmid20833164en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage248en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.cryobiol.2010.09.001
dc.identifier.urihttps://hdl.handle.net/20.500.12395/24824
dc.identifier.volume61en_US
dc.identifier.wosWOS:000285215600003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBaşpınar, Nuri
dc.institutionauthorÇoyan, Kenan
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofCryobiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBovine semenen_US
dc.subjectFreezingen_US
dc.subjectSperm parametersen_US
dc.subjectDNA damageen_US
dc.subjectLipid peroxidationen_US
dc.subjectAntioxidant activityen_US
dc.titleEffects of Antioxidants on Post-thawed Bovine Sperm and Oxidative Stress Parameters: Antioxidants Protect DNA Integrity Against Cryodamageen_US
dc.typeArticleen_US

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