Interleukin-i Beta, Tumor Necrosis Factor-Alpha Levels and Neutrophil Elastase Activity in Peri-İmplant Crevicular Fluid - Correlation With Clinical Parameters and Effect of Smoking

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Küçük Resim

Tarih

2002

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

WILEY

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

The aim of this study was to determine interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) levels and neutrophil elastase (NE) activity in peri-implant crevicular fluid (PICF) of smoker and nonsmoker patients, and to investigate their relationships with clinical parameters. A total of 42 endosseous root-form dental implants of 14 patients were clinically examined by modified Plaque index (PI), modified Gingival index (Gl) and probing depth (PD). Smoking habits of the patients were recorded. PICF of implants were collected by Periopaper strips and IL-1beta, TNF-alpha levels were determined by enzyme-linked immunosorbent assay (ELISA). NE was analyzed with a neutrophil specific chromogenic substrate, N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide. The cytokine and enzyme levels in PICF were expressed as total amount/activity and as concentrations. NE activity in PICF significantly correlated with GI and PD, and IL-1beta levels with GI and PICF volume (P < 0.05). The correlations were stronger when the PICF levels were expressed as total IL-1beta amount and as total NE activity. The implants with inflamed gingiva (GI > 1) had higher levels of IL-1beta and NE activity than implants with noninflamed or slightly inflamed gingiva (GI less than or equal to 1) (P < 0.05). Total NE activity in implants with deep pockets (PD > 3 mm) was greater than the implants with shallow pockets (PD :5 3 mm) (P < 0.05). The implants of smoker patients had significantly lower PICF NE activity and IL-1beta levels, and significantly higher TNF-alpha levels than the implants of nonsmokers (P < 0.05). The findings of the present study indicate that NE activity and IL-1beta levels in PICF may be used to measure implant health status of patients who do not smoke.

Açıklama

Anahtar Kelimeler

crevicular fluid, elastase, interleukin-1, peri-implantitis, tumor necrosis factor

Kaynak

Clinical Oral Implants Research

WoS Q Değeri

Q1

Scopus Q Değeri

Q1

Cilt

13

Sayı

5

Künye

Ataoğlu, H., Alptekin, N. Ö., Haliloğlu, S., Gürsel, M., Ataoğlu, T., Serpek, B., Durmuş, E., (2002). Interleukin-i Beta, Tumor Necrosis Factor-Alpha Levels and Neutrophil Elastase Activity in Peri-İmplant Crevicular Fluid - Correlation With Clinical Parameters and Effect of Smoking. Clinical Oral Implants Research, 13(5), 470-476. Doi: 10.1034/j.1600-0501.2002.130505.x