Matrix Metalloproteinases 2 and 9 Activity in Bovine Synovial Fluids
Yükleniyor...
Dosyalar
Tarih
2000
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wıley
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Matrix metalloproteinases (MMPs) are important enzymes found in connective tissues and thought to be involved in cartilage degradation. They are detectable in bovine synovial fluid and may play a destructive role in bovine septic arthritis. The MMP gelatinase enzymes were detected by gelatin zymography using image analysis of the gels. The active gelatinase levels were determined by a gelatin degradation enzyme-linked immunosorbent assay (ELISA). Increased concentrations of MMP-9 activity were found in the synovial fluids of cows with septic arthritis (P < 0.001) in comparison with fluids from normal joints. Using the gelatin degradation ELISA the net active gelatinases were measured, and significant increases were found in gelatinase bioactivities in synovial fluids from septic joint disease cases (P < 0.001). Increased concentrations of MMP-2 activity were found in the synovial fluids of cows with aseptic arthritis, which appeared to be playing an important role in degradation of articular cartilage in joint disease. This finding required further investigation.
Açıklama
Anahtar Kelimeler
Kaynak
Journal of Veterinary Medicine Series a-physiology Pathology Clinical Medicine
WoS Q Değeri
N/A
Scopus Q Değeri
N/A
Cilt
47
Sayı
8
Künye
Arıcan, M., Carter, S. D., Clegg, D., Coughlan, A. R., (2000). Matrix Metalloproteinases 2 and 9 Activity in Bovine Synovial Fluids. Journal of Veterinary Medicine Series a-physiology Pathology Clinical Medicine, 47(8), 449-456. Doi: 10.1046/j.1439-0442.2000.00291.x
