DSpace Arşivi :: by Yazar "Traş, Bünyamin" değerine göre listeleniyor

Yazar "Traş, Bünyamin" seçeneğine göre listele

Listeleniyor 1 - 20 / 44

Alpha lipoic acid and vitamin E improve atorvastatin-induced mitochondrial dysfunctions in rats
(Elsevier B.V., 2020) Eser Faki, Hatice; Traş, Bünyamin; Üney, Kamil
To determine the effects of alpha lipoic acid (ALA) and vitamin E (Vit E) on mitochondrial dysfunction caused by statins. A total of 38 Wistar Albino rats were used in this study. The control group received dimethyl sulfoxide. The atorvastatin (A) group received atorvastatin (10 mg/kg). The A + ALA group received atorvastatin (10 mg/kg) and ALA (100 mg/kg). The A + Vit E group was administered atorvastatin (10 mg/kg) and Vit E (100 mg/kg). The A + ALA + Vit E group was administered atorvastatin (10 mg/kg), ALA (100 mg/kg) and Vit E (100 mg/kg). All applications were administered simultaneously by gavage for 20 days. ATP level and complex I activity were measured from liver, muscle, heart, kidney and brain. Atorvastatin significantly decreased the ATP levels in heart and kidney, while a slight decrease was seen in liver, muscle and brain. Atorvastatin caused an insignificant decrease in the complex I activity in all tissues examined. ALA administration significantly improved the ATP levels in the liver, heart and kidney, while Vit E improved the ATP levels in all tissues except the muscle compared to Atorvastatin group. Single administration of both ALA and vit E ameliorated complex I activity in the muscle, heart, kidney and brain. The combination of ALA and Vit E significantly improved the ATP levels in the liver, heart, kidney and brain and also provided significant improvements the complex I activity in all tissues. The undesirable effects of Atorvastatin on mitochondrial functions in this study ameliorated by using ALA and/or Vit E alone and in combination.
Bazı Antimikrobiyel I?laçların Plazma ve Lenf Sıvısındaki Farmakokinetik Profillerinin Karşılaştırılması
(1999) Elmas, Muammer; Traş, Bünyamin
Some pharmacokinetic parameters and concentrations of four antimicrobial agents in plasma and lymph fluids were compared for determination of penetration into peripheral tissues. Eighteen healthy, adult sheep (Turk Merino x Hampshire cross, 18-24 month, weighing 32-37 kg) were used. For collection of lymph samples, the efferent vessel of NI, cervicalis superficialis sinister was cannulated with a polyethylene catheter. All antimicrobial agents were administered intramuscularly at single recommended doses (Chloramphenicol 30 mg/kg b.wt., Enrofloxacin 2.5 mg/kg b. wt., Sulphadoxine-trimethoprim 16 mg/kg b.wt). Subsequently, blood and lymph samples were concurrently obtained at 2, 4, 8, 12, 16 and 24 hr postinjection. Concentrations of these agents in all samples were analysed by HPLC. Concentrations of enrofloxacin in lymph fluids at all sampling times were found to be higher than plasma (p<0.01), but concentrations of sulphadoxine in plasma and lymph fluids at all sampling times were not found to be statistically different (p>0.05). The level of chloramphenicol in plasma was found to be higher than lymph fluid only at 2 hr (p<0.05). Concentration of trimethoprim in lymph fluids was found to be higher than plasma at 2 hr (p<0.02), but the level of lymph fluid was found to be lower than plasma at 4 hr after IM administration (p<0.002). However, levels of chloramphenicol and trimetoprim in plasma and lymph fluids were found to be similar at other sampling times. Ratios of lymph AUC(total) / AUC(total) of chloramphenicol, enrofloxacin, sulphadoxine and trimethoprim were found to be 0.97, 1.37, 0.96 and 0.86, respectively. While differences between lymph AUC(total) and plasma AUC(total) of enrofloxacin were found to be statistically significant (p<0.003), the AUC(total) of other drugs in plasma and lymph fluids were found not to be significant (p>0.05). While terminal elimination half-lives (t 1/2?) of agents in plasma were found to be 2.47, 3.35, 3.94 and 2.39, the same parameters of agents in lymph fluids were found to be 2.30, 3.73, 4.01 and 2.85, respectively. There were no significant differences between these parameters of all agents (p>0.05). The results show that when enrofloxacin, sulphadoxine and trimethoprim were used at recommended doses and intervals, these drugs penetrated peripheral tissues quickly and at sufficient concentrations, and chloramphenicol also penetrated quickly into peripheral tissue, but this dosages regimen was inadequate to supply effective concentrations in tissues.
Behaviours of drugs in the milk - A review
(Ataturk Universitesi, 2018) Ozdemir, Zeynep; Traş, Bünyamin
Milk is a food containing many biologically active substances that have an important place in the nourishment of newborns and adults. The transition of the drugs used in the treatment, as well as the environmental pollutants to milk cause a potential risk for consumer health as well as economical losses due to exceed of the legal limits of these compounds set by authorities. The transition of these chemicals to milk is complex; while active transport and passive diffusion play were found to have an important role. The transition abilities of the drugs into milk are defined by milk/plasma ratio. The M/P ratios of the drugs are affected by the composition of the milk and the physicochemical properties of the drug. The concentration of the drugs in the milk depends on the factors of the drug (protein binding, ionization, molecular weight, lipophilicity, drug-drug and drug-nutrient interactions) and organism (race, species, lactation period, parity, disease and nutrition). If the transition properties of the compounds of concern are known or able to be modelized in kinetic applications, it can be useful for preventing milk from drug residues.The success of mastitis treatment depends on the proper use of drugs and knowing of the behaviour of drugs in the milk. © Ataturk Universitesi. All rights reserved.
Comparative Pharmacokinetics of Enrofloxacin and Tissue Concentrations of Parent Drug and Ciprofloxacin After Intramuscular Administrations of Free and Liposome-encapsulated Enrofloxacin in Rabbits
(BLACKWELL VERLAG GMBH, 2002) Elmas, Muammer; Yazar, Enver; Baş, A.L.; Traş, Bünyamin; Bayezit, M.; Yapar, K.
Pharmacokinetic properties and tissue concentrations of enrofloxacin and ciprofloxacin were compared after intramuscular (i.m.) administrations of free and liposome-encapsulated enrofloxacin at the dose of 5 mg/kg body weight (bw). Twelve healthy adult New Zealand white rabbits were used in the experiment. Blood samples were obtained at 10, 20, 40, 60 and 90 min and 2, 4, 6, 8 and 12 h and tissue samples were collected 24 h after injection. Concentrations of drugs in serum were determined by high-performance liquid chromatography. Pharmacokinetics were best described by a two-compartment open model. Results indicated that absorption rate was slow, peak concentration was higher (P<0.05), and the time to peak concentration (t(max) congruent to 1.5 h) was significantly longer (P<0.05) for liposome-encapsulated enrofloxacin (LEE) when compared with free enrofloxacin. Values of elimination half-life (t(1/2beta)=12.9 h) and mean residence time (MRT=17.6 h) of liposome-encapsulated enrofloxacin were longer (P<0.05) and total clearance (Cl = 0.43 l/h/kg) was lower than those of free form. Moreover, the distribution volume at steady-state (V-d(ss)=14.4 l/kg) of enrofloxacin administered encapsulated into liposomes was significantly higher (P<0.05) than that of free enrofloxacin (FE). The tissue levels of enrofloxacin and ciprofloxacin after LEE injection were not different (P>0.05) from FE. In conclusion, the result of present study suggest that LEE may be a beneficial and valuable formulation in the treatment of infectious diseases caused by sensitive pathogens in animals, providing sustained drug release from injection side and prolonged therapeutic serum concentrations after i.m. administration.
Determination of Intracellular (Neutrophil and Monocyte) Concentrations of Free and Liposome Encapsulated Ampicillin in Sheep
(Czech Academy Agricultural Sciences, 2006) Yazar, Enver; Baş, Ahmet Levent; Birdane, Yavuz Osman; Yapar, Kürşad; Elmas, Muammer; Traş, Bünyamin
In the current study, intracellular ( neutrophil and monocyte) concentrations of free and liposome encapsulated ampicillin in sheep were investigated. Free ampicillin ( 5 mg/kg b.w.) and liposome encapsulated ampicillin ( 5 mg/kg b.w.) were administered as a bolus intravenous injection to sheep. After the injections, blood samples ( 5 ml) were collected into tubes from v. jugularis at 10, 30, 60 minutes and 2, 4 and 8 hours. Neutrophils and monocytes were isolated, and lysed in distilled water. Ampicillin concentrations were measured by high performance liquid chromatography. The results indicate that liposome encapsulated ampicillin caused the higher intracellular concentrations within neutrophil ( ratio of liposome encapsulated ampicillin/free ampicillin; from 1.393 to 5.416) and monocyte ( ratio of liposome encapsulated ampicillin/free ampicillin; from 0.973 to 2.906) cells than free ampicillin, and liposome encapsulated ampicillin existed a longer length of time within neutrophil ( 4 hours) and monocyte ( 4 hours) cells than free ampicillin ( 60 minutes), as well. This formulation may be beneficial, in that the treatment of intracellular infections are caused by sensitive bacteria.
Effect Of Acute Ruminal Acidosis On Riboflavin and Niacin Concentrations In Sheep
(Selçuk Üniversitesi, 1993) Başoğlu, Abdullah; Turgut, Kürşat; Eksen, Mursayettin; Traş, Bünyamin; Maden, Mehmet; Ok, Mahmut; Baş, A. Levent; Keçeci, Tufan
Six nonlactating, nonpregnant adult ewes divided into two groups of 3 animals each as a control and experimental groups were used to evaluate the effect of experimentally induced acute ruminal acidosis on plasma and rumen fluid riboflavin and niacin concentrations. Decreasements in ruminal fluid pH, the count of protozoa and increase in the count of bacteria were related to gradual decreases in plasma riboflavin and niacin concentration. Plasma riboflavin and niacin concentrations decreased respectively from 9.51 mcg/ ml and 7 01 mcg/ml to 0.42 mcg/ml and 0.09mcg / m * l at the first day of the experiment (p < 0.05) and than inclined towards normal values. Similar drastical decrease in the niacin concentration of rumen fluid was determined at the first day of the experiment.
The effect of furosemide and acetazolamide induced ion trapping phenomena on renal excretion of chemicals
(1998) Traş, Bünyamin; Ok, Mahmut; Elmas, Muammer; Şen, İsmail; Baş, Ahmet Levent; Tanyıldızı, Sadettin
Bu deneysel çalışma, zorunlu diürezis ile vücuttan atılımı artırmak için kimyasalların pKa değerine göre diüretik ilaç seçiminin önemini ortaya koymak üzere gerçekleştirildi. Denemede, ağırlıkları 15-20 kg arasında değişen 15 adet köpek kullanıldı. Köpekler Grup I, II ve III olmak üzere üç gruba ayrıldı ve her gruptaki köpeklere 1 ml'sinde 200 mg sülfadoksin ve 40 mg trimetoprim içeren Animar (Roche)'dan İM yolla yüksek dozda injekte edildi. Grup Il'deki hayvanlara Animar enjeksiyonundan 2 saat sonra İM yolla 5 mg/kg dozunda furosemid uygulandı. Üçüncü gruptaki hayvanlara ise Animar uygulamasını takibeden 1. ve 8. saatlerde toplam 200 mg dozunda asetazolamid ikiye bölünerek oral yolla verildi. Toplanan kan ve idrar numunelerindeki sülfadoksin ve trimetoprim konsantrasyonları HPLC yardımı ile belirlendi. Asetazolamidin sülfadoksinin, furosemidin ise trimetoprimin böbrekten atılımı üzerinde daha etkili olduğu gözlendi. Asetazolamid idrar pH'sını önemli, oranda artırırken, furosemid çok az derecede bir düşüşe neden oldu. Bu çalışmanın sonuçları, kimyasalların böbrekten atılımı üzerine, diüretiklerin potansiyel etkilerinden daha çok diğer faktörlerin etkili olabileceğini ortaya koydu.
The effect of N-acetylcysteine on the treatment of clinical endometritis and pregnancy rate in dairy cows
(2014) Traş, Bünyamin; Dinç, Dursun A.; Üney, Kamil
Amaç: Bu çalışmada süt ineklerinde klinik endometritisin tedavisi ve gebelik oranı üzerine N-asetilsistein (NAS)'in etkisinin belirlenmesi amaçlandı. Gereç ve Yöntem: Çalışma, ticari bir süt işletmesinde 50-60 günlük gönüllü bekleme süresinden sonra endometritisin klinik bulgularını gösteren 36 inek üzerinde gerçekleştirildi. Klinik endometritis, vajinada belirlenen uterus akıntısının değerlendirilmesiyle tanımlandı. İneklerden servikal svap örnekleri mikroorganizma ve tedavi için en etkili antibakteriyel ajanı belirlemek için toplandı. İnekler rastgele NAS tedavisi yapılan (NAS-T) ve yapılmayan (nNAS-T) olmak üzere iki gruba ayrıldı. NAS-T grubuna (n18) %2'lik NAS uygulamasından 12 saat sonra amoksisilin trihidratpotasyum klavulanat (3500 mg875 mg) uygulaması yapıldı ve bu uygulama üç gün tekrar edildi. nNAS-T grubuna (n18), NAS-T grubunda uygulanan tedavi protokolündeki NAS uygulaması yerine 100 mL serum fizyolojik uygulaması yapıldı. Tedavi oranı, tedaviyi takiben gerçekleşen ilk doğal östrusta klinik endometritisin bulgularını göstermeyen (vaginal muayenede şeffaf veya yarı şeffaf mukus) ineklerin oranı olarak tanımlandı. Hayvanlar tedaviden sonraki ilk doğal östrusta suni yolla tohumlandı. Bulgular: NAS-T grubunda tedavi (%83.3) ve gebelik (%66.7) oranlarının nNAS-T grubuna (sırasıyla %55.5 ve %27.8) göre önemli derecede yüksek olduğu belirlendi (P0.05). Öneri: İneklerde klinik endometritisin tedavisinde NAS kullanımı, tedavi başarısını ve gebelik oranını artırabilir.
Effect of Phlorhizin-Induced Ketosis on Riboflavin and Niacin Levels in Sheep
(Selçuk Üniversitesi, 1993 Ocak) Başoğlu, Abdullah; Turgut, Kürşat; Eksen, Mursayettin; Traş, Bünyamin; Maden, Mehmet; Ok, Mahmut; Baş, A. Levent; Keskin, Ercan
Six non-lactating, non-pregnant adult ewes divided into two groups of 3 animal each as a control and experimental groups were used to evaluate the effect of phlorhizin-induced ketosis on plasma and rumen fluid riboflavin and niacin concentrations. The mean pH values and the counts of protozoa and bacteria of the rumen fluid in experimental group did not change significantly (p > 0.05) Alteration of plasma riboflavin concentration in experimental group was not significant (p > 0.05) . Whereas both plasma and rumen fluid niacin concentrations decreased from 5.03mcg / m * l and 9.53 mcg / m * l to 2.39mcg / m * l and 2.41 mcg/ml respectively and decraements in both plasma and rumen fluid niacin concentrations were significant (p<0.05).
Effect of Tilmicosin on Cardiac Muscle and Serum Creatine Kinases Activities and Serum Total Protein Level in Healthy Male Balb/C Mice
(ECOLE NATIONALE VETERINAIRE TOULOUSE, 2001) Yazar, Enver; Altunok, Vahdettin; Elmas, Muammer; Traş, Bünyamin; Baş, Ahmet Levent; Özdemir, Vural
In this study, the effect of tilmicosin on cardiac muscle and serum creatine kinases activities and serum total protein level was investigated. Forty male Balb/C mice were used as materials. Ten mice were used as a control group, and thirty mice were injected with tilmicosin (25 mg/kg body weight, SC, single injection) and monitored for 3 days. The results obtained in the present study show that use of tilmicosin caused temporary increases in cardiac muscle creatine kinase activity and serum total protein level in male Balb/C mice.
The Effect of Tilmicosin on Cardiac Superoxide Dismutase and Glutathione Peroxidase Activities
(Blackwell Verlag Gmbh, 2002) Yazar, Enver; Altunok, Vahdettin; Elmas, Muammer; Traş, Bünyamin; Baş, Ahmet Levent; Özdemir, V.
In this study, the effect of tilmicosin on cardiac superoxide dismutase and glutathione peroxidase activities was investigated. Forty male BALB/c mice were used as material. Ten mice served as a control group, and 30 mice were injected with tilmicosin (25 mg/kg body weight. subcutaneously. with a single injection). After drug administration, they were monitored for 3 days. Tilmicosin caused decreases in cardiac superoxide dismutase and glutathione peroxidase activities.
Effect of Tilmicosin on Serum Creatine Kinase, Creatine Kinase-MB and Troponin I Levels in New Zealand White Rabbits
(Eugen Ulmer Gmbh CO, 2002) Yazar, Enver; Birdane, Yavuz Osman; Elmas, Muammer; Traş, Bünyamin; Baş, Ahmet Levent
In this study, the effect of tilmicosin on creatine kinase, creatine kinase-MB and troponin I levels were investigated to determine the possible cardiotoxic effect. Ten male White New Zealand rabbits were used as materials and tilmicosin was injected at a dose of 25 mg/kg body weight, subcutanously, by single injection. Blood samples were taken before the injection (=control) and at 6 hours after injection. Tilmicosin caused increases in creatine kinase, creatine kinase-MB and troponin I levels. However, especially histopathological examinations have to be made for fully determination of tilmicosin cardiotoxicity in rabbits.
Effects of Continuous Supplementations of Ascorbic Acid, Aspirin, Vitamin E and Selenium on Some Haematological Parameters and Serum Superoxide Dismutase Level in Broiler Chickens
(Carfax Publishing, 2000) Traş, Bünyamin; İnal, Fatma; Baş, Ahmet Levent; Altunok, Vahdettin; Elmas, Muammer; Yazar, Enver
1. This study was conducted using male broiler chickens to determine the effects of ascorbic acid, aspirin, ascorbic acid+aspirin, vitamin E+selenium and ascorbic acid+aspirin+vitamin E+selenium supplementations on haematological parameters and serum superoxide dismutase concentration. 2. One hundred and twenty day-old male Hubbunt broiler chicks were randomly divided into 6 experimental groups of 20 chicks each and placed in different pens. Groups 2, 3, 4, 5 and 6 were given a diet supplemented with ascorbic acid, aspirin (in water), ascorbic acid+aspirin, vitamin E+selenium and ascorbic acid+aspirin+vitamin E+selenium, respectively for 45 d while group 1 was given a commercial broiler diet. 3. There was no significant effect of ascorbic acid, aspirin, ascorbic acid+aspirin, vitamin E+selenium supplementations on any of the haematological parameters (red blood cell, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin concentration, mean corpuscular haemoglobin) in broilers but ascorbic acid+aspirin+vitamin E+selenium supplementation significantly decreased the white blood cell counts. 4. In addition to this, ascorbic acid, aspirin, ascorbic acid+aspirin and ascorbic acid+aspirin+vitamin E+selenium supplementations had no significant effect on the serum superoxide dismutase level, but vitamin E+selenium supplementation increased the serum superoxide dismutase level.
Effects of Continuous Supplementations of Ascorbic Acid, Aspirin, Vitamin E, and Selenium on Performance, Immune Response, and Some Biochemical Parameters Under Normal Environmental and Management Conditions in Broilers
(EUGEN ULMER GMBH CO, 2001) Traş, Bünyamin; İnal, Fatma; Baş, Ahmet Levent; Altunok, Vahdettin; Elmas, Muammer; Yazar, Enver
Male broiler chickens were used to determine the effects of ascorbic acid, aspirin, ascorbic acid + aspirin, vitamin E + selenium, and ascorbic acid + aspirin + vitamin E + selenium supplementation on performance, IgG levels, and some biochemical parameters in blood. Three hundred and ninety six 1-day-old male. Hubbunt broilers were randomly divided into six groups. Each group was placed in different pens and feed and water were provided ad libitum. Performance parameters (body weight, feed intake and feed conversion), IgG levels, vitamins A and E, and some biochemical parameters in blood (Na, K, Cl, total protein and albumin) were measured on days 15, 30, and 45 in all groups. The results revealed at the end of the study showed that aspirin, vitamin C, vitamin E + selenium administrations exhibited no effects on levels of vitamin A, vitamin E, total protein, albumin, Na, Cl and K in blood, while aspirin administration decreased IgG level. Vitamin C administration increased IgG level in serum, aspirin + vitamin C administration increased albumin and K in serum, aspirin + vitamin C + vitamin E + selenium administration increased vitamin E and Na level in serum. Also, there was no significant difference in performance between the control and the experimental groups. It was suggested that vitamin C administration to diets may be useful for broiler breeds due to the observed increased IgG level.
Effects of Drugs on Kinetic Values of Cytokines, Adenosine Deaminase and 13,14-dihydro-15-keto-prostaglandin F? in Endotoxemia: A Different Approach 2
(2010) Altan, Feray; Elmas, Muammer; Er, Ayşe; Üney, Kamil; Çetin, Gül; Traş, Bünyamin; Yazar, Enver
Amaç: Lipopolisakkaritle (LPS) oluşturulan deneysel endotoksemide sitokinler, adenozin deaminaz (ADA) ve 13,14-dihidro-15-keto-prostaglandin F2? (PGM)’nın kinetik değerlerine tek başlarına ve/veya kombine uygulanan enrofloksasin (ENR), fluniksin meglumin (FM) ve deksametazonun (DEX) etkilerini belirlemektir. Gereç ve Yöntem: Araştırmada kullanılan ratlar 7 gruba ayrıldı. Deneysel endotoksemi oluşturmak için pozitif kontrol grubu dahil bütün gruplara LPS uygulandı. Diğer altı gruba ENR, FM, düşük doz DEX, yüksek doz DEX, ENR+FM+düşük doz DEX ve ENR+FM+yüksek doz DEX uygulandı. Uygulama sonrası 0, 1, 2, 4, 6, 8, 12, 24 ve 48. saatlerde kan örnekleri toplandı. Tümör nekroz faktör alfa (TNF?), interlökin-6 (IL6), interlökin-10 (IL-10), ADA ve PGM düzeyleri ELISA ile belirlendi. Eğri altında kalan alan (EAA0-48) farmakokinetik programla, plazma veya serum maksimum konsantrasyon (Cmax) ile maksimum konsantrasyona ulaşma zamanı (tmax) direk bakı yöntemiyle belirlendi. Bulgular: Pozitif kontrol (LPS) grubuyla kıyaslandığında EAA0-48 değerlerinin; ENR grubunda PGM için artarken (p<0.05), IL-6, IL-10 ve ADA için azaldığı (p<0.05); FM grubunda IL-6 ve ADA’ya özgü olarak küçüldüğü (p<0.05); DEX tek başına veya kombine uygulandığı gruplarda da azaldığı (p<0.05) belirlendi. Öneri: Farklı örnekleme zamanlarında çok sayıda ölçülen aynı endotoksemi belirteçlerinin toplu değerlendirilmesinde kinetik parametrelerden özellikle EAA’nin farklı ve akılcı bir yaklaşım olarak dikkate alınabileceği kanaatine varıldı.
Effects of Drugs Used in Endotoxic Shock on Oxidative Stress and Organ Damage Markers
(TAYLOR & FRANCIS LTD, 2010) Yazar, Enver; Er, Ayşe; Üney, Kamil; Bülbül, Aziz; Avcı, Gülcan Erbil; Elmas, Muammer; Traş, Bünyamin
The aim of this study was to determine the effects of enrofloxacin (ENR), flunixin meglumine (FM) and dexamethasone (DEX) on antioxidant status and organ damage markers in experimentally-induced endotoxemia. Rats were divided into three groups. To induce endotoxemia, lipopolysaccharide (LPS) was injected into all groups, including the positive control. The two other groups received the following drugs (simultaneously with LPS): ENR + FM + low-dose DEX and ENR + FM + high-dose DEX. After the treatments, blood samples were collected at 0, 1, 2, 4, 6, 8, 12, 24 and 48 h. Oxidative stress parameters were determined by ELISA, while serum organ damage markers were measured by autoanalyser. LSP increased (p < 0.05) malondialdehyde, 13,14-dihydro-15-keto-prostaglandin F-2 alpha and nitric oxide, while LPS reduced vitamin C. These changes were especially inhibited (p < 0.05) by ENR + FM + high-dose DEX. LPS increased organ damages markers. Cardiac and hepatic damage was not completely inhibited by any treatment, whereas renal damage was inhibited by two treatments. This study suggested that ENR + FM + high-dose DEX is most effective in the LPS-caused oxidative stress and organ damages.
Effects of Enrofloxacin, Flunixin and Dexamethasone on Indicators of Oxidative and Organ Damage in Lipopolysaccharide-Induced Endotoxemia
(Medwell Online, 2010) Er, Ayşe; Altan, Feray; Çetin, Gül; Üney, Kamil; Traş, Bünyamin; Elmas, Muammer; Yazar, Enver
The aim of this study was to determine the effects of enrofloxacin., flunixin meglumine and dexamethasone on antioxidant status and markers of organ damage in endotoxemia. Rats were divided into four groups. The groups received the following drugs (simultaneously with lipopolysaccharide): enrofloxacin, flunixin meglumine, low-dose dexamethasone or high-dose dexamethasone, respectively. After the treatments, serum and plasma samples were collected at 1, 2, 4, 6, 8, 12, 24 and 48 h. The levels of malondialdehyde, nitric oxide, superoxide dismutase, vitamin C and 13,14-dihydro-15-keto-prostaglandin F-2 alpha were determined with ELISA. The cardiac, hepatic and renal damage markers were measured with autoanalyzer. Elevated levels of malondialdehyde were relatively inhibited by high-dose dexamethasone. Increases in the levels of nitric oxide were inhibited by low and high-dose dexamethasone while increases in the level of 13,14-dihydro-15-keto prostaglandin F-2 alpha were inhibited by all treatments except enrofloxacin. No treatments inhibited the decrease in vitamin C levels. Cardiac and hepatic damage was not inhibited completely whereas renal damage was inhibited by treatment with low or high-dose dexamethasone. The results show that although low-dose dexamethasone had antioxidant activity and protected against organ damage, high-dose dexamethasone may be more beneficial in the treatment of endotoxemia.
Effects of Enrofloxacin, Flunixin Meglumine and Dexamethasone on Disseminated Intravascular Coagulation, Cytokine Levels and Adenosine Deaminase Activity in Endotoxaemia in Rats
(Akademiai Kiado Rt, 2010) Yazar, Enver; Bülbül, Aziz; Avcı, Gülcan Erbil; Er, Ayşe; Üney, Kamil; Elmas, Muammer; Traş, Bünyamin
The aim of this study was to determine the effects of drugs used in the treatment of endotoxaemia on disseminated intravascular coagulation, cytokine levels and adenosine deaminase activities in endotoxaemic rats. Rats were divided into seven groups. Lipopolysaccharide (LPS) was injected into all groups, including the positive control group. The other six groups received the following drugs: enrofloxacin (ENR), flunixin meglumine (FM), low-dose dexamethasone (DEX), high-dose DEX, ENR + FM + low-dose DEX, and ENR + FM + high-dose DEX. After the treatments, serum and plasma samples were collected at 0, 1, 2, 4, 6, 8, 12, 24 and 48 hours (h). A coagulometer was used to determine the levels of coagulation values, while ELISA was used to assay serum cytokines and adenosine deaminase (ADA). Low-dose DEX alone and combined treatments depressed the levels of cytokines and ADA (from 371 to 70 IU/L at 6 h) significantly and inhibited the decrease of coagulation values (antithrombin from 67 to 140% at 6 h, fibrinogen from 54 to 252 mg/dL at 6 h). In summary, FM + high-dose DEX may be the preferred treatment of endotoxaemia because of its highest effectiveness. FM plus high-dose DEX may be a new therapy for endotoxaemic domestic animals.
Efficacies of Free and Liposome-Encapsulated Enrofloxacin (Baytril®) Against Staphylococcus Aureus Infection in Turkish Shepherd Dog Neutrophils in Vitro
(2000) Baş, Ahmet Levent; Elmas, Muammer; Şimşek, Atilla; Traş, Bünyamin; Yazar, E.; Hadimli, H. H.
The influence of free and liposome-encapsulated enrofloxacin at concentrations of 1, 2 and 4 ?g/ml was studied on phagocytic and intracellular killing of Staphylococcus aureus, in cultured neutrophils of six healthy Turkish Shepherd Dogs. Enrofloxacin was encapsulated by using reverse-phase evaporation method in multilamellar liposomes composed of 15 mg egg phosphatidylcholine and 35 mg cholesterol. Phagocytic and killing activity of neutrophils were determined by fluorescent microscopic technique. Liposome-encapsulated enrofloxacin significantly enhanced the intraphagocytic killing of bacteria in neutrophils compared to free drug. Phagocytic activity of neutrophils was not significantly different among groups. The results obtained in the present study show that use of enrofloxacin encapsulated in liposomes may improve the antibacterial efficacy of the antibiotic.
Enrofloksasi?ni?n Sütten Atılma Süresi? Üzeri?ne Deneysel Çalışma
(Selçuk Üniversitesi, 1993) Traş, Bünyamin; Baş, A. Levent; Oğuz, Halis
Enrofloxacin was given to clinically healty six holstein dairy cows at a dosage of 2, 5mg / k * g of body weight by IM as only dose. Milk samples were taken from cows 24th, 48th, 72th, 96th and 120th hours after drug administration and before administration. Residues of enrofloxacin were determined in milk samples by HPLC method. Determined means concentrations of enrofloxacin in milk samles were respectively 0.035 ± 0.005, 0.025 ± 0.009, 0.0054± 3mcg / m * l at 24th, 48th and 72th hours. Enrofloxacin was not determined in milk samples at 96th and 120th hours.

Yazar "Traş, Bünyamin" seçeneğine göre listele

Sayfa Başına Sonuç

Sıralama seçenekleri